------------------------------------------------------------ Publication Information ------------------------------------------------------------ Title: Experimental studies on microscopic, multiphase transport phenomena: Novel applications of light sheet fluorescence microscopy Keywords: LSFM, SPIM, PLIF, mass transfer Authors: Lotta Kursula - 0009-0006-6587-2709 Institute of Multiphase Flows, Hamburg University of Technology, Hamburg, Germany Felix Kexel - 0000-0003-4268-2348 Institute of Multiphase Flows, Hamburg University of Technology, Hamburg, Germany Marko Hoffmann Institute of Multiphase Flows, Hamburg University of Technology, Hamburg, Germany Niklas-Maximilian Epping - 0000-0002-7348-4566 Institute of Technical Biocatalysis, Hamburg University of Technology, Hamburg, Germany Paul Bubenheim - 0000-0001-6954-4274 Institute of Technical Biocatalysis, Hamburg University of Technology, Hamburg, Germany Koichi Terasaka - 0000-0002-9978-5463 Department of Applied Chemistry, Faculty of Science an Technology, Keio University, Yokohama, Japan Andreas Liese - 0000-0002-4867-9935 Institute of Technical Biocatalysis, Hamburg University of Technology, Hamburg, Germany Michael Schlüter - 0000-0001-5969-2150 Institute of Multiphase Flows, Hamburg University of Technology, Hamburg, Germany DOI of publication: https://doi.org/10.1007/s12650-026-01115-7 DOI of data supplement: https://doi.org/10.15480/882.16054 License: Public Domain Mark 1.0 Universal Abstract of the paper: Over the past two decades, light sheet fluorescence microscopy has developed to a powerful tool for studies of dynamics in biological systems. In a new development, we apply light sheet fluorescence microscopy as a novel experimental measurement technology within the field of multiphase process engineering and fluid dynamics. The technology enables a number of novel studies of single- and multiphase transport phenomena on a microscopic scale. In the current publication, we introduce the first implementation of light sheet fluorescence microscopy in the field and demonstrate its applicability on one exemplary measurement of diffusive oxygen mass transfer from an oxygen bubble to degassed water. The results prove that such measurements can be conducted with a high spatial resolution with a submicron pixel pitch and enable precise studies on microscopic transport phenomena. Besides measurements of mass transfer, on which we lay the focus here, light sheet fluorescence microscopy further enables studies of fluid dynamics on a microscopic scale. ------------------------------------------------------------ Data Information ------------------------------------------------------------ 01_Data_measurements This folder contains the raw images (.tiff, 12-bit) from the LIF measurements introduced in the publication. ID1/2/3 refers to the measurement ID (3 measurements in total) 02_Data_calibration This folder contains the raw images (.tiff, 12-bit) used for the calibration of the captured fluorescence intensity to oxygen concentration.