This README_Supplementary_Data.txt file was generated on 2026-08-13 by Sri Sannihita Chavali (sri.chavali@tuhh.de) ------------------- GENERAL INFORMATION ------------------- Name: Sri Sannihita Chavali (ORCID: 0009-0008-7662-0974) Role/Function: Data Collector, Data Curator (main contact person) Institution: Institute of Technical Microbiology, Hamburg University of Technology Address: Kasernenstraße 12, 21073 Hamburg, Germany Email: sri.chavali@tuhh.de Name: Yitian Zhou (ORCID: 0000-0001-8378-8320) Role/Function: Data Collector Institution: Institute of Technical Microbiology, Hamburg University of Technology Address: Kasernenstraße 12, 21073 Hamburg, Germany Name: Jan Nissen (ORCID: 0009-0004-2915-6890) Role/Function: Data Collector/Contributing Researcher Institution: Heinrich-Blasius-Institute, Hamburg University of Applied Sciences Address: Berliner Tor 21, 20099 Hamburg, Germany Name: Janek R. Weiler (ORCID: 0000-0003-4930-5764) Role/Function: Data Collector/Contributing Researcher Institution: Institute of Technical Microbiology, Hamburg University of Technology Address: Kasernenstraße 12, 21073 Hamburg, Germany Name: Christian Jonas Lapp (ORCID: 0000-0002-8631-1218) Role/Function: Data Collector/Contributing Researcher Institution: Institute of Technical Microbiology, Hamburg University of Technology Address: Kasernenstraße 12, 21073 Hamburg, Germany Name: Maike Orth (ORCID: 0000-0002-0220-7705) Role/Function: Contributing Researcher Institution: Institute of Solids Process Engineering and Particle Technology, Hamburg University of Technology Address: Denickestraße 15, 21073 Hamburg, Germany Name: Stefan Heinrich (ORCID: 0000-0002-7901-1698) Role/Function: Contributing Researcher Institution: Institute of Solids Process Engineering and Particle Technology, Hamburg University of Technology Address: Denickestraße 15, 21073 Hamburg, Germany Name: Alexandra von Kameke (ORCID: 0000-0002-1913-774X) Role/Function: Contributing Researcher Institution: Heinrich-Blasius-Institute, Hamburg University of Applied Sciences Address: Berliner Tor 21, 20099 Hamburg, Germany Name: Miriam Edel-Teichmann (ORCID: 0009-0008-6283-0006) Role/Function: Contributing Researcher Institution: Institute of Technical Microbiology, Hamburg University of Technology Address: Kasernenstraße 12, 21073 Hamburg, Germany Name: Johannes Gescher (ORCID: 0000-0002-1625-8810) Role/Function: Principal Investigator (alternative contact person) Institution: Institute of Technical Microbiology, Hamburg University of Technology Address: Kasernenstraße 12, 21073 Hamburg, Germany Email: johannes.gescher@tuhh.de Date of data collection: Ferbruary 2024 - April 2026 Location of data collection: Institute of Technical Microbiology, Hamburg University of Technology, Hamburg, Germany Funding: This project is funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – SFB 1615 – 503850735. --------------------------- SHARING/ACCESS INFORMATION --------------------------- Title of data set: "Training Cupriavidus necator for growth on cathode surfaces: impact of adaptive evolution for genomic alterations leading to accelerated biofilm development" DOI of dataset: https://doi.org/10.15480/882.17914 Handle: https://hdl.handle.net/11420/64347 Keywords: Adaptive Laboratory Evolution, Microbial electrosynthesis, --------------------- DATA & FILE OVERVIEW --------------------- ### 01_Progenitor_biovolume_growth.csv (creation date: 13.08.2026, version: 01) - **Description:** Progression of biovolume for the progenitor strain over the course of the experiment. - **Columns:** - Time: Time of the experiment in days - Biovolume: Measured biovolume in µl/cm2 - Normalised biovolume: Normalized biovolume in mm3/mm2 - Avg: Average normalized biovolume over time - Std Dev: Standard deviation of the biovolume ### 02_29strains_consortium_afterALE_biovolume_growth.csv (creation date: 13.08.2026, version: 01) - **Description:** Progression of biovolume for the 29-strain consortium after Adaptive Laboratory Evolution (ALE). - **Columns:** - Time: Time of the experiment in days - Biovolume: Measured biovolume in µl/cm2 - Normalised biovolume: Normalized biovolume in mm3/mm2 - Avg: Average normalized biovolume over time - Std Dev: Standard deviation of the biovolume ### 03_strain890_biovolume_growth.csv (creation date: 13.08.2026, version: 01) - **Description:** Progression of biovolume for strain 890 over the course of the experiment. - **Columns:** - Time: Time of the experiment in days - Biovolume: Measured biovolume in µl/cm2 - Normalised biovolume: Normalized biovolume in mm3/mm2 - Avg: Average normalized biovolume over time - Std Dev: Standard deviation of the biovolume ### 04_Porosity_estimation_progenitor_JG2015.csv (creation date: 13.08.2026, version: 01) - **Description:** Porosity estimations for the progenitor strain JG2015 biofilm. - **Columns:** - Time: Time of the experiment in days - Biofilm height from height maps: Measured biofilm height in mm - Avg: Average biofilm height over triplicates - Std Dev: Standard deviation of the height - Biofilm height from biovolume: Calculated biofilm height from biovolume in mm and µm - Porosity: porosity of the biofilm over the triplicate over the course of the experiment ### 05_Porosity_estimation_29strain_consortium_afterALE.csv (creation date: 13.08.2026, version: 01) - **Description:** Porosity estimations for the 29-strain consortium biofilm after Adaptive Laboratory Evolution (ALE). - **Columns:** - Time: Time of the experiment in days - Biofilm height from height maps: Measured biofilm height in mm - Avg: Average biofilm height over triplicates - Std Dev: Standard deviation of the height - Biofilm height from biovolume: Calculated biofilm height from biovolume in mm and µm - Porosity: porosity of the biofilm over the triplicate over the course of the experiment ### 06_Porosity_estimation_strain890.csv (creation date: 13.08.2026, version: 01) - **Description:** Porosity estimations for the strain 890 biofilm. - **Columns:** - Time: Time of the experiment in days - Biofilm height from height maps: Measured biofilm height in mm - Avg: Average biofilm height over triplicates - Std Dev: Standard deviation of the height - Biofilm height from biovolume: Calculated biofilm height from biovolume in mm and µm - Porosity: porosity of the biofilm over the triplicate over the course of the experiment ### 07_genetic_variants.csv (creation date: 13.08.2026, version: 01) - **Description:** List of identified genetic variants and mutations across the sequenced strains. ### 08_pairwise_comparison.csv (creation date: 13.08.2026, version: 01) - **Description:** Pairwise genetic and phenotypic comparisons between the different strains and consortiums. ----------------- RESEARCH CONTEXT ----------------- This study investigates enhancement of cathodic biofilm formation by Cupriavidus necator under electroautotrophic conditions, utilizing an adaptive laboratory evolution (ALE) approach in a microfluidic oxic microbial electrosynthesis (oMES) platform, and compares a rapidly colonizing, genetically robust evolved isolate (strain 890) to its progenitor strain for sustainable CO₂ valorisation.