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  4. Oxygen-dependent redox control enables growth-decoupled biotransformations in Cupriavidus necator
 
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Oxygen-dependent redox control enables growth-decoupled biotransformations in Cupriavidus necator

Citation Link: https://doi.org/10.15480/882.16935
Type
Dataset
Version
v1.0
Date Issued
2026-04-07
Author(s)
Jesenofsky, Vivien  
Technische Mikrobiologie V-7  
Researcher
Gescher, Johannes  
Technische Mikrobiologie V-7  
Edel, Miriam
Technische Mikrobiologie V-7  
Data Curator
Jesenofsky, Vivien  
Technische Mikrobiologie V-7  
Data Collector
Jesenofsky, Vivien  
Technische Mikrobiologie V-7  
Weiler, Janek  
Technische Mikrobiologie V-7  
Contact
Gescher, Johannes  
Technische Mikrobiologie V-7  
Language
English
DOI
https://doi.org/10.15480/882.16935
TORE-URI
https://hdl.handle.net/11420/62473
Used equipment
HPLC-Anlage High Performance Liquid Chromatograph.  
Abstract
This dataset contains measurements from growth and cell suspension experiments using engineered Cupriavidus necator strains for oxygen-dependent biotransformations. Experiments include: Acetoin reduction to 2,3-butanediol under ±O₂; Glycerol conversion to 1,3-propanediol under ±O₂; Growth experiments under oxic conditions; Long-term viability assays with live/dead staining. Oxygen availability was used as a control parameter to modulate intracellular redox metabolism and direct NADH utilization toward either product formation or respiration.
Subjects
Cupriavidus necator
redox metabolism
oxygen limitation
acetoin
2,3-butanediol
1,3-propanediol
HPLC
DDC Class
660.6: Biotechnology
Funding(s)
SFB 1615 - Teilprojekt C02: SMARTe kontinuierliche bioelektrochemische Prozesse  
Funding Organisations
Deutsche Forschungsgemeinschaft (DFG)  
More Funding Information
This project is funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – SFB 1615 – 503850735.
License
https://creativecommons.org/publicdomain/mark/1.0/
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01_HPLC_data_cellsuspension_plusO2.csv

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02_HPLC_data_cellsuspension_minusO2.csv

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03_HPLC_data_Growth_experiment_plusO2.csv

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04_HPLC_data_cellsuspension_Live_Dead.csv

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2.68 KB

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05_HPLC_cellsuspension_Propanediol.csv

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1.5 KB

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CSV

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