M03.007 Determination of free carbohydrates by HPLC-RI

For the determination of free carbohydrates and their degradation products, such as cellobiose, glucose, xylose, arabinose, HMF and furfural, aqueous samples are centrifuged and analysed by an HPLC system coupled to a refractive index detector. Separation is performed with a Polymer-based HPLC phase column which combines steric exclusion, ligand exchange and partition effects.