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Protein-protein-interaction network organization of the hypusine modification system
Citation Link: https://doi.org/10.15480/882.1937
Publikationstyp
Journal Article
Publikationsdatum
2012-08-10
Sprache
English
Enthalten in
Volume
11
Issue
11
Start Page
1289
End Page
1305
Citation
Molecular & cellular proteomics : MCP 11 (11): 1289-1305 (2012)
Publisher DOI
Scopus ID
Publisher
American Society for Biochemistry and Molecular Biology, HighWire Press
Hypusine modification of eukaryotic initiation factor 5A (eIF-5A) represents a unique and highly specific post-translational modification with regulatory functions in cancer, diabetes, and infectious diseases. However, the specific cellular pathways that are influenced by the hypusine modification remain largely unknown. To globally characterize eIF-5A and hypusine-dependent pathways, we used an approach that combines large-scale bioreactor cell culture with tandem affinity purification and mass spectrometry: "bioreactor-TAP-MS/MS." By applying this approach systematically to all four components of the hypusine modification system (eIF-5A1, eIF-5A2, DHS, and DOHH), we identified 248 interacting proteins as components of the cellular hypusine network, with diverse functions including regulation of translation, mRNA processing, DNA replication, and cell cycle regulation. Network analysis of this data set enabled us to provide a comprehensive overview of the protein-protein interaction landscape of the hypusine modification system. In addition, we validated the interaction of eIF-5A with some of the newly identified associated proteins in more detail. Our analysis has revealed numerous novel interactions, and thus provides a valuable resource for understanding how this crucial homeostatic signaling pathway affects different cellular functions.
Schlagworte
Animals
Computational Biology
DNA-Binding Proteins
Humans
Lysine
Mass Spectrometry
Mice
Mixed Function Oxygenases
Multivesicular Bodies
NIH 3T3 Cells
Nuclear Proteins
Oxidoreductases Acting on CH-NH Group Donors
Peptide Fragments
Peptide Initiation Factors
Protein Transport
RNA-Binding Proteins
Recombinant Fusion Proteins
Reproducibility of Results
Ribosomal Proteins
Subcellular Fractions
Protein Interaction Maps
Protein Processing, Post-Translational
DDC Class
570: Biowissenschaften, Biologie
610: Medizin
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