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Construction and verification of the transcriptional regulatory response network of Streptococcus mutans upon treatment with the biofilm inhibitor carolacton
Citation Link: https://doi.org/10.15480/882.1201
Other Titles
Konstruierung und Verifizierung eines Transkriptionsregulationsnetzwerks von Streptococcus mutans in Reaktion auf die Behandlung mit dem Biofilminhibitor Carolacton
Publikationstyp
Doctoral Thesis
Date Issued
2014
Sprache
English
Author(s)
Advisor
Title Granting Institution
Technische Universität Hamburg
Place of Title Granting Institution
Hamburg
Examination Date
2014-10-23
Institut
TORE-DOI
Streptococcus mutans is a biofilm forming oral pathogen primarily responsible for causing human dental caries. To infer the underlying network mediating the growth inhibitory effect of a newly identified secondary metabolite carolacton on S. mutans biofilms, the transcriptional regulatory response network (TRRN) of S. mutans biofilms upon carolacton treatment was constructed using a systems biology approach combining time-resolved transcriptomic data, reverse engineering, transcription factor binding sites, and experimental validation. From the inferred TRRN, predicted transcriptional regulatory interactions were confirmed using interaction studies.
Sensitivity testing of deletion mutants of key regulators from the predicted network identified the cysteine metabolism regulator CysR as being essential for the response of S. mutans biofilms to carolacton.
Sensitivity testing of deletion mutants of key regulators from the predicted network identified the cysteine metabolism regulator CysR as being essential for the response of S. mutans biofilms to carolacton.
Subjects
Streptococcus mutans ; Karies ; Biofilm-Inhibitor Carolacton ; Transkriptom ; Transkriptionsregulationsnetzwerk ; Schlüsselregulator
Streptococcus mutans ; Caries ; Biofilm inhibitor-Carolacton ; Transcriptome ; Transcriptional regulatory network ; Key regulators
DDC Class
570: Biowissenschaften, Biologie
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