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Untersuchungen zur Enantioselektivität Thiamindiphosphat abhängiger Enzyme : reaktionstechnische Optimierung der C-C Bindungsbildung
Citation Link: https://doi.org/10.15480/882.871
Other Titles
Investigations on asymmetric syntheses by thiamine diphosphate dependent enzymes : optimization of C-C bond formation by reaction engineering
Publikationstyp
Doctoral Thesis
Date Issued
2010
Sprache
German
Author(s)
Advisor
Title Granting Institution
Technische Universität Hamburg
Place of Title Granting Institution
Hamburg
Examination Date
2010-08-12
Institut
TORE-DOI
Benzoylformate Decarboxylase from Pseudomonas putida is a thiamine diphosphate dependent enzyme that catalyzes the assymetric C-C bond formation of (S)-2-hydroxypropiophenon. In this work the effect of hydrostatic pressure up to 250 MPa and pH is shown on the enantioselectivity for the recombinant wtBFD as well as the variants BFD F464I, BFD A460I and BFD A460I F464I. The reversibility of the system was investigated as well as inhibitory studies with methylbenzoylphosphonate derivatives by spectroscopic methods, activity determination and X ray chrystallography.
Subjects
Thiamindiphosphat
Thiaminpyrophosphat
Benzoylformiatdecarboxylase
asymmetrische Synthese
Enantioselektivität
Enzyminhibierung
thiamine diphosphate
benzoylformate decarboxylase
asymmetric synthesis
inhibition
enantioselectivity
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