Replication of Polymer-Based Peptide Microarrays by Multi-Step Transfer

Publikationstyp
Journal Article
Date Issued
2016-09-01
Sprache
English
Author(s)
Striffler, Jakob  
Mattes, Daniela S.
Schillo, Sebastian
Münster, Bastian
Palermo, Andrea
Ridder, Barbara
Welle, Alexander  
Trouillet, Vanessa  
Stadler, Volker  
Markovic, Goran
Proll, Günther
Bräse, Stefan
Loeffler, Felix F.  
Nesterov-Müller, Alexander
Breitling, Frank  
TORE-URI
https://hdl.handle.net/11420/59164
Journal
ChemNanoMat  
Volume
2
Issue
9
Start Page
897
End Page
903
Citation
ChemNanoMat 2 (9): 897-903 (2016)
Publisher DOI
10.1002/cnma.201600194
Scopus ID
2-s2.0-85033226921
Publisher
Wiley
Peptide microarrays serve as a high-throughput tool for the readout of protein–peptide interactions. We present a multi-step purification of high-complexity peptide microarrays by transfer to specialized surfaces. Only correctly synthesized peptides are transferred from the synthesis surface to various substrates where they can be immobilized by a variety of different anchor molecules. Here, we demonstrate the transfer to rigid streptavidin, epoxide, gold, and azide surfaces. The reported method reduces the cost of purified peptide array production through the reproduction of one array several times.
Subjects
high-throughput screening
label-free detection
peptide array
replication
DDC Class
600: Technology