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Increase of protein yield in high five cells in a single-use perfusion bioreactor by medium replacement
Publikationstyp
Journal Article
Date Issued
2012-11-21
Sprache
English
Author(s)
Institut
TORE-URI
Journal
Volume
85
Issue
1-2
Start Page
111
End Page
117
Citation
Chemie-Ingenieur-Technik 1-2 (85): 111-117 (2013)
Publisher DOI
Scopus ID
Publisher
Wiley-VCH Verlag
A multitude of heterologous proteins is needed in pharmaceutical research. These have to be produced in robust and flexible cultivation systems to allow a fast and cost-effective handling. Moreover, these systems should be scalable and meet quality and regulatory requirements. The baculovirus expression vector system propagated on High Five insect cells is a popular choice for this purpose. For this expression system an efficient, robust and scalable process was developed based on a single-use technology and a medium replacement strategy with cell retention. It is shown that a medium replacement in the Wave bioreactor is feasible and can be scaled up to a 10-L scale without much effort..
Subjects
High Five cells
Insect cells
Medium replacement
Perfusion
Single-use bioreactor
Wave bioreactor
DDC Class
570: Biowissenschaften, Biologie