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The molecular toolbox for chromosomal heterologous multiprotein expression in Escherichia coli
Publikationstyp
Conference Paper
Date Issued
2012-11-21
Sprache
English
Author(s)
Journal
Volume
40
Issue
6
Start Page
1222
End Page
1226
Citation
Biochemical Society Transactions 40 (6): 1222-1226 (2012-12-01)
Publisher DOI
Scopus ID
PubMed ID
23176458
Publisher
Portland Press
Heterologous multiprotein expression is the tool to answer a number of questions in basic science as well as to convert strains into producers and/or consumers of certain compounds in applied sciences. Multiprotein expression can be driven by plasmids with the disadvantages that the gene dosage might, in some cases, lead to toxic effects and that the continuous addition of antibiotics is undesirable. Stable genomic expression of proteins can forgo these problems and is a helpful and promising tool in synthetic biology. In the present paper, we provide an extract of methods from the toolbox for chromosome-based heterologous expression in Escherichia coli. ©The Authors Journal compilation.
Subjects
Counter-selection
Escherichia coli
Heterologous multiprotein expression
Phage
Promoter
Recombineering
DDC Class
570: Biowissenschaften, Biologie