Automatisierte Probenfiltration zur Analyse intrazellulärer Metaboliten bei höheren Biomassekonzentrationen

Other Titles
Automated fast filtration for the analysis of intracellular metabolites at higher biomass concentrations
Publikationstyp
Journal Article
Date Issued
2022-01-12
Sprache
German
Author(s)
Hans, Enrico  
Zeng, An-Ping  orcid-logo
Institut
Bioprozess- und Biosystemtechnik V-1  
TORE-DOI
10.15480/882.4441
TORE-URI
http://hdl.handle.net/11420/12981
Journal
Chemie - Ingenieur - Technik  
Volume
94
Issue
6
Start Page
864
End Page
872
Citation
Chemie-Ingenieur-Technik 94 (6): 864-872 (2022-06-01)
Publisher DOI
10.1002/cite.202100091
Scopus ID
2-s2.0-85122656609
Publisher
Wiley-VCH Verl.
Studies of intracellular metabolites of Corynebacterium glutamicum mostly were done at levels of biomass concentrations below 10 g L−1. In this work we could show that it is also possible up to 32 g L−1 biomass concentration. Hereby we could point towards a faster filtration process with a modification of the already established automated fast filtration. We also could quantify the influence of a genetic modification of the lysine feedback inhibited asparto kinase in the aspartate metabolism with the automated fast filtration at higher biomass concentrations.
Subjects
Corynebacterium glutamicum
Fast filtration
Intracellular metabolites
Lysin biosynthesis
Intrazelluläre Metaboliten
Lysinbiosynthese
Schnellfiltration
DDC Class
600: Technik
Publication version
publishedVersion
Lizenz
https://creativecommons.org/licenses/by-nc/4.0/
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