A simple and fast method to analyze the orientation of c-type cytochromes in the outer membrane of Gram-negative bacteria

Publikationstyp
Journal Article
Date Issued
2010-05-06
Sprache
English
Author(s)
Richter, Katrin  
Bücking, Clemens  
Schicklberger, Marcus  
Gescher, Johannes 
TORE-URI
http://hdl.handle.net/11420/14674
Journal
Journal of microbiological methods  
Volume
82
Issue
2
Start Page
184
End Page
186
Citation
Journal of Microbiological Methods 82 (2): 184-186 (2010-08-01)
Publisher DOI
10.1016/j.mimet.2010.04.011
Scopus ID
2-s2.0-77954085077
PubMed ID
20451567
Publisher
Elsevier
Outer membrane cytochromes catalyze the final reduction step of respiratory chains to electron acceptors that cannot diffuse through the outer membrane of Gram-negative bacteria. We developed an in vivo method to detect the orientation of outer membrane cytochromes via analysis of electron transfer reactions between these enzymes and riboflavin.
Subjects
Localization
Outer membrane cytochromes
Riboflavin
DDC Class
570: Biowissenschaften, Biologie