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A simple and fast method to analyze the orientation of c-type cytochromes in the outer membrane of Gram-negative bacteria
Publikationstyp
Journal Article
Date Issued
2010-05-06
Sprache
English
Volume
82
Issue
2
Start Page
184
End Page
186
Citation
Journal of Microbiological Methods 82 (2): 184-186 (2010-08-01)
Publisher DOI
Scopus ID
PubMed ID
20451567
Publisher
Elsevier
Outer membrane cytochromes catalyze the final reduction step of respiratory chains to electron acceptors that cannot diffuse through the outer membrane of Gram-negative bacteria. We developed an in vivo method to detect the orientation of outer membrane cytochromes via analysis of electron transfer reactions between these enzymes and riboflavin.
Subjects
Localization
Outer membrane cytochromes
Riboflavin
DDC Class
570: Biowissenschaften, Biologie