Recyclable and Robust Optical Nanoprobes with Engineered Enzymes for Sustainable Serodiagnostics

Publikationstyp
Journal Article
Date Issued
2023-11-23
Sprache
English
Author(s)
Liu, Yuxin
Wei, Zheng  
Damian, Matteo  
Zhu, Xingjun
Knaus, Tanja
Zhang, Hong
Mutti, Francesco G.
Loeffler, Felix F.  
TORE-URI
https://hdl.handle.net/11420/59158
Journal
Advanced materials  
Volume
35
Issue
47
Article Number
2306615
Citation
Advanced Materials 35 (47): 2306615 (2023)
Publisher DOI
10.1002/adma.202306615
Scopus ID
2-s2.0-85174287157
ISSN
09359648
Recyclable fluorescence assays that can be stored at room temperature would greatly benefit biomedical diagnostics by bringing sustainability and cost-efficiency, especially for point-of-care serodiagnostics in developing regions. Here, a general strategy is proposed to generate recyclable fluorescent probes by using engineered enzymes with enhanced thermo-/chemo-stability, which maintains an outstanding serodiagnostic performance (accuracy >95%) after 10 times of recycling as well as after storage at elevated temperatures (37 °C for 10 days). With these three outstanding properties, recyclable fluorescent probes can be designed to detect various biomarkers of clinical importance by using different enzymes.
Subjects
detection
lanthanides
nanoparticles
point-of-care
DDC Class
600: Technology