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Publisher DOI: 10.1186/s13068-021-01981-3
Title: Extracellular riboflavin induces anaerobic biofilm formation in Shewanella oneidensis
Language: English
Authors: Edel, Miriam 
Sturm, Gunnar 
Sturm-Richter, Katrin 
Wagner, Michael 
Novion Ducassou, Julia 
Couté, Yohann 
Horn, Harald 
Gescher, Johannes 
Keywords: Biofilm;Current density;Microbiology;Quorum sensing;Shewanella oneidensis
Issue Date: 3-Jun-2021
Publisher: BioMed Central
Source: Biotechnology for Biofuels 14 (1): 130 (2021-12)
Journal: Biotechnology for biofuels 
Abstract (english): 
Background: Some microorganisms can respire with extracellular electron acceptors using an extended electron transport chain to the cell surface. This process can be applied in bioelectrochemical systems in which the organisms produce an electrical current by respiring with an anode as electron acceptor. These organisms apply flavin molecules as cofactors to facilitate one-electron transfer catalyzed by the terminal reductases and in some cases as endogenous electron shuttles.

Results: In the model organism Shewanella oneidensis, riboflavin production and excretion trigger a specific biofilm formation response that is initiated at a specific threshold concentration, similar to canonical quorum-sensing molecules. Riboflavin-mediated messaging is based on the overexpression of the gene encoding the putrescine decarboxylase speC which leads to posttranscriptional overproduction of proteins involved in biofilm formation. Using a model of growth-dependent riboflavin production under batch and biofilm growth conditions, the number of cells necessary to produce the threshold concentration per time was deduced. Furthermore, our results indicate that specific retention of riboflavin in the biofilm matrix leads to localized concentrations, which by far exceed the necessary threshold value.

Conclusion: This study describes a new quorum-sensing mechanism in S. oneidensis. Biofilm formation of S. oneidensis is induced by low concentrations of riboflavin resulting in an upregulation of the ornithine-decarboxylase speC. The results can be applied for the development of strains catalyzing increased current densities in bioelectrochemical systems.
DOI: 10.15480/882.3756
ISSN: 1754-6834
Document Type: Article
Funded by: Bundesministerium für Bildung und Forschung (BMBF) 
More Funding information: This work was supported by a grant of the Bundesministerium für Bildung und Forschung (BMBF), No. 031B0847A. Proteomic experiments were partly supported by the ProFI Grant (ANR-10-INBS-08-01).
License: CC BY 4.0 (Attribution) CC BY 4.0 (Attribution)
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