DC FieldValueLanguage
dc.contributor.authorSturm, Gunnar-
dc.contributor.authorBrunner, Stefanie-
dc.contributor.authorSuvorova, Elena-
dc.contributor.authorDempwolff, Felix-
dc.contributor.authorReiner, Johannes Eberhard-
dc.contributor.authorGraumann, Peter-
dc.contributor.authorBernier-Latmani, Rizlan-
dc.contributor.authorMajzlan, Juraj-
dc.contributor.authorGescher, Johannes-
dc.date.accessioned2021-09-02T05:57:49Z-
dc.date.available2021-09-02T05:57:49Z-
dc.date.issued2018-12-01-
dc.identifier.citationApplied and Environmental Microbiology 84 (23): e02208-18 (2018-12-01)de_DE
dc.identifier.issn0099-2240de_DE
dc.identifier.urihttp://hdl.handle.net/11420/10240-
dc.description.abstractChromate is one of the major anthropogenic contaminants on Earth. Leucobacter chromiiresistens is a highly chromate-resistant strain, tolerating chromate concentrations in LB medium of up to 400 mM. In response to chromate stress, L. chromiiresistens forms biofilms, which are held together via extracellular DNA. Inhibition of biofilm formation leads to drastically decreased chromate tolerance. Moreover, chromate is reduced intracellularly to the less-toxic Cr(III). The oxidation status and localization of chromium in cell aggregates were analyzed by energy-dispersive X-ray spectroscopy coupled to scanning transmission electron microscopy and X-ray absorption spectroscopy measurements. Most of the heavy metal is localized as Cr(III) at the cytoplasmic membrane. As a new cellular response to chromate stress, we observed an increased production of the carotenoid lutein. Carotenoid production could increase membrane stability and reduce the concentration of reactive oxygen species. Bioinformatic analysis of the L. chromiiresistens genome revealed several gene clusters that could enable heavy-metal resistance. The extreme chromate tolerance and the unique set of resistance factors suggest the use of L. chromiiresistens as a new model organism to study microbial chromate resistance.en
dc.language.isoende_DE
dc.relation.ispartofApplied and environmental microbiologyde_DE
dc.subjectChromate resistancede_DE
dc.subjectHeavy metalsde_DE
dc.subjectHeavy-metal resistancede_DE
dc.subjectLeucobacterde_DE
dc.titleChromate resistance mechanisms in Leucobacter chromiiresistensde_DE
dc.typeArticlede_DE
dc.type.diniarticle-
dcterms.DCMITypeText-
tuhh.abstract.englishChromate is one of the major anthropogenic contaminants on Earth. Leucobacter chromiiresistens is a highly chromate-resistant strain, tolerating chromate concentrations in LB medium of up to 400 mM. In response to chromate stress, L. chromiiresistens forms biofilms, which are held together via extracellular DNA. Inhibition of biofilm formation leads to drastically decreased chromate tolerance. Moreover, chromate is reduced intracellularly to the less-toxic Cr(III). The oxidation status and localization of chromium in cell aggregates were analyzed by energy-dispersive X-ray spectroscopy coupled to scanning transmission electron microscopy and X-ray absorption spectroscopy measurements. Most of the heavy metal is localized as Cr(III) at the cytoplasmic membrane. As a new cellular response to chromate stress, we observed an increased production of the carotenoid lutein. Carotenoid production could increase membrane stability and reduce the concentration of reactive oxygen species. Bioinformatic analysis of the L. chromiiresistens genome revealed several gene clusters that could enable heavy-metal resistance. The extreme chromate tolerance and the unique set of resistance factors suggest the use of L. chromiiresistens as a new model organism to study microbial chromate resistance.de_DE
tuhh.publisher.doi10.1128/AEM.02208-18-
tuhh.type.opus(wissenschaftlicher) Artikel-
dc.type.driverarticle-
dc.type.casraiJournal Article-
tuhh.container.issue23de_DE
tuhh.container.volume84de_DE
dc.identifier.pmid30266727-
dc.identifier.scopus2-s2.0-85057872695-
tuhh.container.articlenumbere02208-18de_DE
item.creatorOrcidSturm, Gunnar-
item.creatorOrcidBrunner, Stefanie-
item.creatorOrcidSuvorova, Elena-
item.creatorOrcidDempwolff, Felix-
item.creatorOrcidReiner, Johannes Eberhard-
item.creatorOrcidGraumann, Peter-
item.creatorOrcidBernier-Latmani, Rizlan-
item.creatorOrcidMajzlan, Juraj-
item.creatorOrcidGescher, Johannes-
item.languageiso639-1en-
item.creatorGNDSturm, Gunnar-
item.creatorGNDBrunner, Stefanie-
item.creatorGNDSuvorova, Elena-
item.creatorGNDDempwolff, Felix-
item.creatorGNDReiner, Johannes Eberhard-
item.creatorGNDGraumann, Peter-
item.creatorGNDBernier-Latmani, Rizlan-
item.creatorGNDMajzlan, Juraj-
item.creatorGNDGescher, Johannes-
item.openairetypeArticle-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_6501-
item.mappedtypeArticle-
item.fulltextNo Fulltext-
item.cerifentitytypePublications-
crisitem.author.deptTechnische Mikrobiologie V-7-
crisitem.author.orcid0000-0002-7744-9645-
crisitem.author.orcid0000-0002-0340-4233-
crisitem.author.orcid0000-0002-1625-8810-
crisitem.author.parentorgStudiendekanat Verfahrenstechnik-
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