Please use this identifier to cite or link to this item: https://doi.org/10.15480/882.1962
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DC FieldValueLanguage
dc.contributor.authorRasigraf, Olivia-
dc.contributor.authorSchmitt, Julia-
dc.contributor.authorJetten, Mike S.M.-
dc.contributor.authorLüke, Claudia-
dc.date.accessioned2019-01-16T11:28:05Z-
dc.date.available2019-01-16T11:28:05Z-
dc.date.issued2017-05-23-
dc.identifier.citationMicrobiologyOpen 4 (6): e00475- (2017)de_DE
dc.identifier.issn2045-8827de_DE
dc.identifier.urihttps://tubdok.tub.tuhh.de/handle/11420/1965-
dc.description.abstractThe biological nitrogen cycle is driven by a plethora of reactions transforming nitrogen compounds between various redox states. Here, we investigated the metagenomic potential for nitrogen cycle of the in situ microbial community in an oligotrophic, brackish environment of the Bothnian Sea sediment. Total DNA from three sediment depths was isolated and sequenced. The characterization of the total community was performed based on 16S rRNA gene inventory using SILVA database as reference. The diversity of diagnostic functional genes coding for nitrate reductases (napA;narG), nitrite:nitrate oxidoreductase (nxrA), nitrite reductases (nirK;nirS;nrfA), nitric oxide reductase (nor), nitrous oxide reductase (nosZ), hydrazine synthase (hzsA), ammonia monooxygenase (amoA), hydroxylamine oxidoreductase (hao), and nitrogenase (nifH) was analyzed by blastx against curated reference databases. In addition, Polymerase chain reaction (PCR)-based amplification was performed on the hzsA gene of anammox bacteria. Our results reveal high genomic potential for full denitrification to N2, but minor importance of anaerobic ammonium oxidation and dissimilatory nitrite reduction to ammonium. Genomic potential for aerobic ammonia oxidation was dominated by Thaumarchaeota. A higher diversity of anammox bacteria was detected in metagenomes than with PCR-based technique. The results reveal the importance of various N-cycle driving processes and highlight the advantage of metagenomics in detection of novel microbial key players.en
dc.language.isoende_DE
dc.publisherWileyde_DE
dc.relation.ispartofMicrobiologyOpende_DE
dc.rightsCC BY 4.0de_DE
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.subjectanammoxde_DE
dc.subjectBothnian Seade_DE
dc.subjectdentrificationde_DE
dc.subjectN-cyclede_DE
dc.subjectsedimentde_DE
dc.subject.ddc550: Geowissenschaftende_DE
dc.titleMetagenomic potential for and diversity of N-cycle driving microorganisms in the Bothnian Sea sedimentde_DE
dc.typeArticlede_DE
dc.identifier.urnurn:nbn:de:gbv:830-882.025504-
dc.identifier.doi10.15480/882.1962-
dc.type.diniarticle-
dc.subject.ddccode550-
dcterms.DCMITypeText-
tuhh.identifier.urnurn:nbn:de:gbv:830-882.025504-
tuhh.oai.showtruede_DE
dc.identifier.hdl11420/1965-
tuhh.abstract.englishThe biological nitrogen cycle is driven by a plethora of reactions transforming nitrogen compounds between various redox states. Here, we investigated the metagenomic potential for nitrogen cycle of the in situ microbial community in an oligotrophic, brackish environment of the Bothnian Sea sediment. Total DNA from three sediment depths was isolated and sequenced. The characterization of the total community was performed based on 16S rRNA gene inventory using SILVA database as reference. The diversity of diagnostic functional genes coding for nitrate reductases (napA;narG), nitrite:nitrate oxidoreductase (nxrA), nitrite reductases (nirK;nirS;nrfA), nitric oxide reductase (nor), nitrous oxide reductase (nosZ), hydrazine synthase (hzsA), ammonia monooxygenase (amoA), hydroxylamine oxidoreductase (hao), and nitrogenase (nifH) was analyzed by blastx against curated reference databases. In addition, Polymerase chain reaction (PCR)-based amplification was performed on the hzsA gene of anammox bacteria. Our results reveal high genomic potential for full denitrification to N2, but minor importance of anaerobic ammonium oxidation and dissimilatory nitrite reduction to ammonium. Genomic potential for aerobic ammonia oxidation was dominated by Thaumarchaeota. A higher diversity of anammox bacteria was detected in metagenomes than with PCR-based technique. The results reveal the importance of various N-cycle driving processes and highlight the advantage of metagenomics in detection of novel microbial key players.de_DE
tuhh.publisher.doi10.1002/mbo3.475-
tuhh.publication.instituteWasserressourcen und Wasserversorgung B-11de_DE
tuhh.identifier.doi10.15480/882.1962-
tuhh.type.opus(wissenschaftlicher) Artikelde
tuhh.institute.germanDVGW-Forschungsstellede
tuhh.institute.englishWasserressourcen und Wasserversorgung B-11de_DE
tuhh.gvk.hasppnfalse-
openaire.rightsinfo:eu-repo/semantics/openAccessde_DE
dc.type.driverarticle-
dc.rights.ccbyde_DE
dc.rights.ccversion4.0de_DE
dc.type.casraiJournal Articleen
tuhh.container.issue4de_DE
tuhh.container.volume6de_DE
tuhh.container.startpagee00475de_DE
dc.rights.nationallicensefalsede_DE
item.fulltextWith Fulltext-
item.creatorOrcidRasigraf, Olivia-
item.creatorOrcidSchmitt, Julia-
item.creatorOrcidJetten, Mike S.M.-
item.creatorOrcidLüke, Claudia-
item.creatorGNDRasigraf, Olivia-
item.creatorGNDSchmitt, Julia-
item.creatorGNDJetten, Mike S.M.-
item.creatorGNDLüke, Claudia-
item.grantfulltextopen-
crisitem.author.deptWasserressourcen und Wasserversorgung B-11-
crisitem.author.orcid0000-0003-0084-4003-
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