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dc.contributor.authorSong, Lifu-
dc.contributor.authorWang, Wei-
dc.contributor.authorConrads, Georg-
dc.contributor.authorRheinberg, Anke-
dc.contributor.authorSztajer, Helena-
dc.contributor.authorReck, Michael-
dc.contributor.authorWagner-Döbler, Irene-
dc.contributor.authorZeng, An-Ping-
dc.date.accessioned2019-02-01T13:01:37Z-
dc.date.available2019-02-01T13:01:37Z-
dc.date.issued2013-06-28-
dc.identifier.citationBMC Genomics 1 (14): 430 (2013)de_DE
dc.identifier.issn1471-2164de_DE
dc.identifier.urihttps://tubdok.tub.tuhh.de/handle/11420/2015-
dc.description.abstractBackground: Mutans streptococci are a group of bacteria significantly contributing to tooth decay. Their genetic variability is however still not well understood.Results: Genomes of 6 clinical S. mutans isolates of different origins, one isolate of S. sobrinus (DSM 20742) and one isolate of S. ratti (DSM 20564) were sequenced and comparatively analyzed. Genome alignment revealed a mosaic-like structure of genome arrangement. Genes related to pathogenicity are found to have high variations among the strains, whereas genes for oxidative stress resistance are well conserved, indicating the importance of this trait in the dental biofilm community. Analysis of genome-scale metabolic networks revealed significant differences in 42 pathways. A striking dissimilarity is the unique presence of two lactate oxidases in S. sobrinus DSM 20742, probably indicating an unusual capability of this strain in producing H2O2 and expanding its ecological niche. In addition, lactate oxidases may form with other enzymes a novel energetic pathway in S. sobrinus DSM 20742 that can remedy its deficiency in citrate utilization pathway.Using 67 S. mutans genomes currently available including the strains sequenced in this study, we estimates the theoretical core genome size of S. mutans, and performed modeling of S. mutans pan-genome by applying different fitting models. An "open" pan-genome was inferred.Conclusions: The comparative genome analyses revealed diversities in the mutans streptococci group, especially with respect to the virulence related genes and metabolic pathways. The results are helpful for better understanding the evolution and adaptive mechanisms of these oral pathogen microorganisms and for combating them. © 2013 Song et al.; licensee BioMed Central Ltd.en
dc.description.sponsorshipBundesministerium für Bildung und Forschungde_DE
dc.language.isoende_DE
dc.publisherBioMed Central ; Springerde_DE
dc.relation.ispartofBMC genomicsde_DE
dc.rightsCC BY 2.0de_DE
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.subjectMutans Streptococcide_DE
dc.subjectStreptococcus Mutansde_DE
dc.subjectStreptococcus Rattide_DE
dc.subjectStreptococcus Sobrinusde_DE
dc.subjectComparative Genomics,de_DE
dc.subjectCore-genomede_DE
dc.subjectPan-genomede_DE
dc.subjectPathogenicityde_DE
dc.subjectLactate Oxidasede_DE
dc.subjectMetabolic Networkde_DE
dc.subject.ddc570: Biowissenschaften, Biologiede_DE
dc.subject.ddc610: Medizinde_DE
dc.titleGenetic variability of mutans streptococci revealed by wide whole-genome sequencingde_DE
dc.typeArticlede_DE
dc.identifier.urnurn:nbn:de:gbv:830-882.026437-
dc.identifier.doi10.15480/882.2012-
dc.type.diniarticle-
dc.subject.ddccode570-
dc.subject.ddccode610-
dcterms.DCMITypeText-
tuhh.identifier.urnurn:nbn:de:gbv:830-882.026437-
tuhh.oai.showtruede_DE
dc.identifier.hdl11420/2015-
tuhh.abstract.englishBackground: Mutans streptococci are a group of bacteria significantly contributing to tooth decay. Their genetic variability is however still not well understood.Results: Genomes of 6 clinical S. mutans isolates of different origins, one isolate of S. sobrinus (DSM 20742) and one isolate of S. ratti (DSM 20564) were sequenced and comparatively analyzed. Genome alignment revealed a mosaic-like structure of genome arrangement. Genes related to pathogenicity are found to have high variations among the strains, whereas genes for oxidative stress resistance are well conserved, indicating the importance of this trait in the dental biofilm community. Analysis of genome-scale metabolic networks revealed significant differences in 42 pathways. A striking dissimilarity is the unique presence of two lactate oxidases in S. sobrinus DSM 20742, probably indicating an unusual capability of this strain in producing H2O2 and expanding its ecological niche. In addition, lactate oxidases may form with other enzymes a novel energetic pathway in S. sobrinus DSM 20742 that can remedy its deficiency in citrate utilization pathway.Using 67 S. mutans genomes currently available including the strains sequenced in this study, we estimates the theoretical core genome size of S. mutans, and performed modeling of S. mutans pan-genome by applying different fitting models. An "open" pan-genome was inferred.Conclusions: The comparative genome analyses revealed diversities in the mutans streptococci group, especially with respect to the virulence related genes and metabolic pathways. The results are helpful for better understanding the evolution and adaptive mechanisms of these oral pathogen microorganisms and for combating them. © 2013 Song et al.; licensee BioMed Central Ltd.de_DE
tuhh.publisher.doi10.1186/1471-2164-14-430-
tuhh.publication.instituteBioprozess- und Biosystemtechnik V-1de_DE
tuhh.identifier.doi10.15480/882.2012-
tuhh.type.opus(wissenschaftlicher) Artikelde
tuhh.institute.germanBioprozess- und Biosystemtechnik V-1de
tuhh.institute.englishBioprozess- und Biosystemtechnik V-1de_DE
tuhh.gvk.hasppnfalse-
openaire.rightsinfo:eu-repo/semantics/openAccessde_DE
dc.type.driverarticle-
dc.rights.ccbyde_DE
dc.rights.ccversion2.0de_DE
dc.type.casraiJournal Articleen
tuhh.container.issue1de_DE
tuhh.container.volume14de_DE
tuhh.container.startpage430de_DE
dc.relation.projectResearch Program “Medical systems biology - MedSys”, project “Development of biofilm inhibitors using a systems biology approach” (0315411)de_DE
dc.rights.nationallicensefalsede_DE
item.fulltextWith Fulltext-
item.creatorGNDSong, Lifu-
item.creatorGNDWang, Wei-
item.creatorGNDConrads, Georg-
item.creatorGNDRheinberg, Anke-
item.creatorGNDSztajer, Helena-
item.creatorGNDReck, Michael-
item.creatorGNDWagner-Döbler, Irene-
item.creatorGNDZeng, An-Ping-
item.creatorOrcidSong, Lifu-
item.creatorOrcidWang, Wei-
item.creatorOrcidConrads, Georg-
item.creatorOrcidRheinberg, Anke-
item.creatorOrcidSztajer, Helena-
item.creatorOrcidReck, Michael-
item.creatorOrcidWagner-Döbler, Irene-
item.creatorOrcidZeng, An-Ping-
item.grantfulltextopen-
crisitem.author.deptBioprozess- und Biosystemtechnik V-1-
crisitem.author.deptBioprozess- und Biosystemtechnik V-1-
crisitem.author.deptBioprozess- und Biosystemtechnik V-1-
crisitem.author.orcid0000-0003-1092-4568-
crisitem.author.orcid0000-0003-2577-5294-
crisitem.author.orcid0000-0001-9768-7096-
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