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Publisher DOI: 10.2174/1874070701711010016
Title: Evaluation of fixed-bed cultures with immobilized Lactococcus Lactis ssp. Lactis on different scales
Language: English
Authors: Faschian, Rebecca 
Eren, Ilyas 
Minden, Steven 
Pörtner, Ralf  
Keywords: fixed-bed reactor;immobilization;lactococcus lactis;scale-up
Issue Date: 28-Jul-2017
Publisher: Bentham Open
Source: Open Biotechnology Journal (11): 16-25 (2017)
Journal or Series Name: The open biotechnology journal 
Abstract (english): Fixed-bed processes, where cells are immobilized within macroporous carriers, are a promising alternative to processes with suspended cells. A scale-up concept is presented in order to evaluate the performance as part of process design of fixed-bed processes. Therefore, Lactococcus lactis cultivation in chemostat and batch mode was compared to fixed bed cultures on three different scales, the smallest being the downscaled Multiferm with 10 mL fixed bed units, the second a 100 mL fixed-bed reactor and the third a pilot scale reactor with 1 L fixed bed volume. As expected, the volume specific lactate productivity of all cultivations was dependent on dilution rate. In suspension chemostat culture a maximum of 2.3 g · L-1·h-1was reached. Due to cell retention in the fixed-beds, productivity increased up to 8.29 g·L-1·h-1at a dilution rate of D = 1.16 h-1(corresponding to 2.4 µmax) on pilot scale. For all fixed bed cultures a common spline was obtained indicating a good scale-up performance.
DOI: 10.15480/882.2105
ISSN: 1874-0707
Institute: Bioprozess- und Biosystemtechnik V-1 
Type: (wissenschaftlicher) Artikel
Funded by: Federal Ministry of Education and Research (BMBF) grant No. 0.31A124A
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