Please use this identifier to cite or link to this item: https://doi.org/10.15480/882.2157
DC FieldValueLanguage
dc.contributor.authorHsu, Hao-Hsiang-
dc.contributor.authorKracht, John-Kevin-
dc.contributor.authorHarder, Laura Elisabeth-
dc.contributor.authorRudnik, Kerstin-
dc.contributor.authorLindner, Gerd-
dc.contributor.authorSchimek, Katharina-
dc.contributor.authorMarx, Uwe-
dc.contributor.authorPörtner, Ralf-
dc.date.accessioned2019-04-01T07:51:43Z-
dc.date.available2019-04-01T07:51:43Z-
dc.date.issued2018-02-02-
dc.identifier.citationJournal of Visualized Experiments 132 (): - (2018)de_DE
dc.identifier.issn1940-087Xde_DE
dc.identifier.urihttp://hdl.handle.net/11420/2243-
dc.description.abstractIn vitro cultivated skin models have become increasingly relevant for pharmaceutical and cosmetic applications, and are also used in drug development as well as substance testing. These models are mostly cultivated in membrane-insert systems, their permeability toward different substances being an essential factor. Typically, applied methods for determination of these parameters usually require large sample sizes (e.g., Franz diffusion cell) or laborious equipment (e.g., fluorescence recovery after photobleaching (FRAP)). This study presents a method for determining permeability coefficients directly in membrane-insert systems with diameter sizes of 4.26 mm and 12.2 mm (cultivation area). The method was validated with agarose and collagen gels as well as a collagen cell model representing skin models. The permeation processes of substances with different molecular sizes and permeation through different cell models (consisting of collagen gel, fibroblast, and HaCaT) were accurately described. Moreover, to support the above experimental method, a simulation was established. The simulation fits the experimental data well for substances with small molecular size, up to 14 x 10-10 m Stokes radius (4,000 MW), and is therefore a promising tool to describe the system. Furthermore, the simulation can considerably reduce experimental efforts and is robust enough to be extended or adapted to more complex setups.en
dc.language.isoende_DE
dc.publisher[S.l.]de_DE
dc.relation.ispartofJoVEde_DE
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.subjectBioengineeringde_DE
dc.subjectIssue 132de_DE
dc.subjectSkin modelde_DE
dc.subjectpermeabilityde_DE
dc.subjectdiffusionde_DE
dc.subjectmembrane insert systemde_DE
dc.subjectsimulationde_DE
dc.subjectfluorescein isothiocyanate-dextrande_DE
dc.subjectfluorescein sodium saltde_DE
dc.subject.ddc570: Biowissenschaften, Biologiede_DE
dc.subject.ddc620: Ingenieurwissenschaftende_DE
dc.titleA method for determination and simulation of permeability and diffusion in a 3D tissue model in a membrane insert system for multi-well platesde_DE
dc.typeArticlede_DE
dc.identifier.urnurn:nbn:de:gbv:830-882.029622-
dc.identifier.doi10.15480/882.2157-
dc.type.diniarticle-
dc.subject.ddccode620-
dc.subject.ddccode570-
dcterms.DCMITypeText-
tuhh.identifier.urnurn:nbn:de:gbv:830-882.029622-
tuhh.oai.showtruede_DE
tuhh.abstract.englishIn vitro cultivated skin models have become increasingly relevant for pharmaceutical and cosmetic applications, and are also used in drug development as well as substance testing. These models are mostly cultivated in membrane-insert systems, their permeability toward different substances being an essential factor. Typically, applied methods for determination of these parameters usually require large sample sizes (e.g., Franz diffusion cell) or laborious equipment (e.g., fluorescence recovery after photobleaching (FRAP)). This study presents a method for determining permeability coefficients directly in membrane-insert systems with diameter sizes of 4.26 mm and 12.2 mm (cultivation area). The method was validated with agarose and collagen gels as well as a collagen cell model representing skin models. The permeation processes of substances with different molecular sizes and permeation through different cell models (consisting of collagen gel, fibroblast, and HaCaT) were accurately described. Moreover, to support the above experimental method, a simulation was established. The simulation fits the experimental data well for substances with small molecular size, up to 14 x 10-10 m Stokes radius (4,000 MW), and is therefore a promising tool to describe the system. Furthermore, the simulation can considerably reduce experimental efforts and is robust enough to be extended or adapted to more complex setups.de_DE
tuhh.publisher.doi10.3791/56412-
tuhh.publication.instituteBioprozess- und Biosystemtechnik V-1de_DE
tuhh.identifier.doi10.15480/882.2157-
tuhh.type.opus(wissenschaftlicher) Artikel-
tuhh.institute.germanBioprozess- und Biosystemtechnik V-1de
tuhh.institute.englishBioprozess- und Biosystemtechnik V-1de_DE
tuhh.gvk.hasppnfalse-
openaire.rightsinfo:eu-repo/semantics/openAccessde_DE
dc.type.driverarticle-
dc.rights.cchttps://creativecommons.org/licenses/by-nc-nd/3.0/de_DE
dc.type.casraiJournal Article-
tuhh.container.issue132de_DE
tuhh.container.startpageArt.-Nr. e56412de_DE
dc.relation.projectEntwicklung und mikrofluidische Charakterisierung eines dynamisch kultivierten Vollhautmodells-
dc.rights.nationallicensefalsede_DE
item.fulltextWith Fulltext-
item.creatorGNDHsu, Hao-Hsiang-
item.creatorGNDKracht, John-Kevin-
item.creatorGNDHarder, Laura Elisabeth-
item.creatorGNDRudnik, Kerstin-
item.creatorGNDLindner, Gerd-
item.creatorGNDSchimek, Katharina-
item.creatorGNDMarx, Uwe-
item.creatorGNDPörtner, Ralf-
item.languageiso639-1en-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_6501-
item.creatorOrcidHsu, Hao-Hsiang-
item.creatorOrcidKracht, John-Kevin-
item.creatorOrcidHarder, Laura Elisabeth-
item.creatorOrcidRudnik, Kerstin-
item.creatorOrcidLindner, Gerd-
item.creatorOrcidSchimek, Katharina-
item.creatorOrcidMarx, Uwe-
item.creatorOrcidPörtner, Ralf-
item.cerifentitytypePublications-
item.grantfulltextopen-
crisitem.author.deptBioprozess- und Biosystemtechnik V-1-
crisitem.author.deptBioprozess- und Biosystemtechnik V-1-
crisitem.author.deptBioprozess- und Biosystemtechnik V-1-
crisitem.author.deptBioprozess- und Biosystemtechnik V-1-
crisitem.author.deptBioprozess- und Biosystemtechnik V-1-
crisitem.author.orcid0000-0003-2336-2058-
crisitem.author.orcid0000-0003-1163-9718-
crisitem.author.parentorgStudiendekanat Verfahrenstechnik-
crisitem.author.parentorgStudiendekanat Verfahrenstechnik-
crisitem.author.parentorgStudiendekanat Verfahrenstechnik-
crisitem.author.parentorgStudiendekanat Verfahrenstechnik-
crisitem.author.parentorgStudiendekanat Verfahrenstechnik-
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