Please use this identifier to cite or link to this item: https://doi.org/10.15480/882.2175
This item is licensed with a CreativeCommons licence https://creativecommons.org/licenses/by/4.0/
Publisher DOI: 10.1038/s41467-018-06156-7
Title: Megahertz serial crystallography
Language: English
Authors: Wiedorn, Max Oliver 
Oberthür, Dominik 
Bean, Richard 
Schubert, Robin 
Werner, Nadine 
Abbey, Brian 
Aepfelbacher, Martin 
Adriano, Luigi 
Allahgholi, A. 
Al-Qudami, Nasser 
Andreasson, Jakob 
Aplin, Steven 
Awel, Salah 
Ayyer, Kartik 
Bajt, Saša 
Barák, Imrich 
Bari, Sadia 
Bielecki, Johan 
Botha, Sabine 
Boukhelef, Djelloul 
Brehm, Wolfgang 
Brockhauser, Sandor 
Cheviakov, Igor 
Coleman, Matthew A. 
Cruz-Mazo, Francisco 
Danilevski, Cyril 
Darmanin, Connie 
Doak, R. Bruce 
Domaracky, Martin 
Dörner, Katerina 
Du, Yang 
Fangohr, Hans 
Fleckenstein, Holger 
Frank, Matthias 
Fromme, Petra 
Gañán-Calvo, Alfonso M. 
Gevorkov, Yaroslav 
Giewekemeyer, Klaus 
Ginn, Helen Mary 
Graafsma, Heinz 
Graceffa, Rita 
Greiffenberg, Dominic 
Gumprecht, Lars 
Göttlicher, Peter 
Hajdu, János 
Hauf, Steffen 
Heymann, Michael 
Holmes, Susannah 
Horke, Daniel A. 
Hunter, Mark S. 
Imlau, Siegfried 
Kaukher, Alexander 
Kim, Yoonhee 
Klyuev, Alexander 
Knoška, Juraj 
Kobe, Bostjan 
Kuhn, Manuela 
Kupitz, Christopher 
Küpper, Jochen 
Lahey-Rudolph, Janine Mia 
Laurus, Torsten 
Le Cong, Karoline 
Letrun, Romain 
Xavier, Paulrajpillai Lourdu 
Maia, Luis 
Maia, Filipe R.N.C. 
Mariani, Valerio 
Messerschmidt, Marc 
Metz, Markus 
Mezza, Davide 
Michelat, Thomas 
Mills, Grant 
Monteiro, Diana C.F. 
Morgan, Andrew J. 
Mühlig, Kerstin 
Munke, Anna 
Münnich, Astrid 
Nette, Julia 
Nugent, Keith A. 
Nuguid, Theresa 
Orville, Allen M. 
Pandey, Suraj 
Pena, Gisel 
Vilanueva-Pérez, Pablo 
Pöhlsen, Jennifer 
Previtali, Gianpietro 
Redecke, Lars 
Riekehr, Winnie Maria 
Rohde, Holger 
Round, Adam 
Safenreiter, Tatiana 
Sarrou, Iosifina 
Sato, Tokushi 
Schmidt, Marius 
Schmitt, Bernd 
Schönherr, Robert 
Schulz, Joachim 
Sellberg, Jonas Alexander 
Seibert, M. Marvin 
Seuring, Carolin 
Shelby, Megan L. 
Shoemann, Robert L. 
Sikorski, Marcin 
Silenzi, Alessandro 
Stan, Claudiu A. 
Shi, Xintian 
Stern, Stephan 
Sztuk-Dambietz, Jolanta 
Szuba, Janusz 
Tolstikova, Aleksandra 
Trebbin, Martin 
Trunk, Ulrich 
Vagovic, Patrik 
Ve, Thomas 
Weinhausen, Britta 
White, Thomas A. 
Wrona, Krzysztof 
Xu, Chen 
Yefanov, Oleksandr 
Zatsepin, Nadia 
Zhang, Jiaguo 
Perbandt, Markus 
Mancuso, Adrian P. 
Betzel, Christian 
Chapman, Henry N. 
Banty, Anton 
Issue Date: 2-Oct-2018
Publisher: Nature Publishing Group UK
Source: Nature Communications 1 (9): 4025 - (2018-10-02)
Journal or Series Name: Nature Communications 
Abstract (english): The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.
URI: http://hdl.handle.net/11420/2300
DOI: 10.15480/882.2175
ISSN: 2041-1723
Institute: Bildverarbeitungssysteme E-2 
Type: (wissenschaftlicher) Artikel
Appears in Collections:Publications (tub.dok)

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