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Publisher DOI: 10.2174/1874120701307010125
Title: Electrical stimulation of NIH-3T3 cells with platinum-PEDOT-electrodes integrated in a bioreactor
Language: English
Authors: Blume, Grit 
Müller-Wichards, Wiebke 
Göpfert, Christiane 
Pörtner, Ralf  
Müller, Jörg 
Keywords: electrical stimulation;cell culture;fibroblasts;integrated biocompatible electrodes;orientation of metaphase plate;polarization index.
Issue Date: 2013
Publisher: Bentham Open
Source: The open biomedical engineering journal 1 (7): 125-132 (2013)
Journal or Series Name: The open biomedical engineering journal 
Abstract (english): The objective of this work involves the development and integration of electrodes for the electrical stimulation of cells within a bioreactor. Electrodes need to fit properties such as biocompatibility, large reversible charge transfer and high flexibility in view of their future application as implants on the tympanic membrane. Flexible thin-film platinum-poly(3,4-ethylene-dioxythiophene)-electrodes on a poly(ethylene terephthalate)-foil manufactured using microsystems technology were integrated into a bioreactor based on the design of a 24 well plate. The murine fibroblast cell line NIH-3T3 was cultured on the foil electrodes and the cells were stimulated with direct voltage and unipolar pulsed voltage. The amplitude, the pulse length and the ratio of pulse to pause were varied. The stimulated cells were stained in order to determine the angle between the cell cleavage plane of the dividing cells and the vector of the electric field. These angles were subsequently used to calculate the polarization index, which is a measure of the orientation of the metaphase plane of dividing cells that occurs for example during wound healing or embryonic morphogenesis.
DOI: 10.15480/882.2186
ISSN: 1874-1207
Institute: Bioprozess- und Biosystemtechnik V-1 
Mikrosystemtechnik E-7 
Type: (wissenschaftlicher) Artikel
Funded by: Bundesministerium für Bildung und Forschung (BMBF).
Project: 01 EZ 0846 
Charakterisierung der Zellwachstumsaktivität von Gewebezellen in 3D-Scaffolds mit integrierten Sensoren 
License: CC BY-NC 3.0 (Attribution-NonCommercial) CC BY-NC 3.0 (Attribution-NonCommercial)
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