Please use this identifier to cite or link to this item: https://doi.org/10.15480/882.2205
DC FieldValueLanguage
dc.contributor.authorPätzold, Magdalena-
dc.contributor.authorWeimer, Anna-
dc.contributor.authorLiese, Andreas-
dc.contributor.authorHoltmann, Dirk-
dc.date.accessioned2019-04-23T12:36:26Z-
dc.date.available2019-04-23T12:36:26Z-
dc.date.issued2019-
dc.identifier.citationBiotechnology report (22): e00333- (2019))de_DE
dc.identifier.issn2215-017Xde_DE
dc.identifier.urihttp://hdl.handle.net/11420/2364-
dc.description.abstractThe Candida rugosa lipase catalyzed esterification of (-)-menthol and lauric acid (LA) was studied in a eutectic mixture formed by both substrates((-)-menthol:LA 3:1, mol/mol). No additional reaction solvent was necessary, since the (-)-menthol:LA deep eutectic solvent (DES) acts as combined reaction medium and substrate pool. Therefore, the esterification is conducted under solvent-free conditions. The thermodynamic water activity (aw) was identified as a key parameter affecting the esterification performance in the (-)-menthol:LA DES. A response surface methodology was applied to optimize the esterification conditions in terms aw, amount of C. rugosa lipase (mCRL) and reaction temperature. Under the optimized reaction conditions (aw = 0.55; mCRL =60 mg; T =45 °C), a conversion of 95 ± 1% LA was achieved (one day), the final (-)-menthyl lauric acid ester concentration reached 1.36 ± 0.04 M (2.25 days). The experimental product formation rate agreed very well with the model prediction.en
dc.description.sponsorshipBMBF: 031B0014Cde_DE
dc.language.isoende_DE
dc.relation.ispartofBiotechnology Reportsde_DE
dc.rightsCC BY-NC-ND 4.0de_DE
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.subjectdeep eutectic solventde_DE
dc.subjectbiocatalysisde_DE
dc.subjectwater activityde_DE
dc.subjectreaction medium engineeringde_DE
dc.subjectresponse surface methodologyde_DE
dc.subjectdesign of experimentsde_DE
dc.subject.ddc500: Naturwissenschaftende_DE
dc.titleOptimization of solvent-free enzymatic esterification in eutectic substrate reaction mixturede_DE
dc.typeArticlede_DE
dc.identifier.urnurn:nbn:de:gbv:830-882.031231-
dc.identifier.doi10.15480/882.2205-
dc.type.diniarticle-
dc.subject.ddccode500-
dcterms.DCMITypeText-
tuhh.identifier.urnurn:nbn:de:gbv:830-882.031231-
tuhh.oai.showtruede_DE
tuhh.abstract.englishThe Candida rugosa lipase catalyzed esterification of (-)-menthol and lauric acid (LA) was studied in a eutectic mixture formed by both substrates((-)-menthol:LA 3:1, mol/mol). No additional reaction solvent was necessary, since the (-)-menthol:LA deep eutectic solvent (DES) acts as combined reaction medium and substrate pool. Therefore, the esterification is conducted under solvent-free conditions. The thermodynamic water activity (aw) was identified as a key parameter affecting the esterification performance in the (-)-menthol:LA DES. A response surface methodology was applied to optimize the esterification conditions in terms aw, amount of C. rugosa lipase (mCRL) and reaction temperature. Under the optimized reaction conditions (aw = 0.55; mCRL =60 mg; T =45 °C), a conversion of 95 ± 1% LA was achieved (one day), the final (-)-menthyl lauric acid ester concentration reached 1.36 ± 0.04 M (2.25 days). The experimental product formation rate agreed very well with the model prediction.de_DE
tuhh.publisher.doi10.1016/j.btre.2019.e00333-
tuhh.publication.instituteTechnische Biokatalyse V-6de_DE
tuhh.identifier.doi10.15480/882.2205-
tuhh.type.opus(wissenschaftlicher) Artikel-
tuhh.institute.germanTechnische Biokatalyse V-6de
tuhh.institute.englishTechnische Biokatalyse V-6de_DE
tuhh.gvk.hasppnfalse-
openaire.rightsinfo:eu-repo/semantics/openAccessde_DE
dc.type.driverarticle-
dc.rights.cchttps://creativecommons.org/licenses/by-nc-nd/4.0/de_DE
dc.type.casraiJournal Article-
tuhh.container.volume24de_DE
tuhh.container.startpagee00333de_DE
dc.rights.nationallicensefalsede_DE
item.fulltextWith Fulltext-
item.languageiso639-1en-
item.creatorGNDPätzold, Magdalena-
item.creatorGNDWeimer, Anna-
item.creatorGNDLiese, Andreas-
item.creatorGNDHoltmann, Dirk-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_6501-
item.creatorOrcidPätzold, Magdalena-
item.creatorOrcidWeimer, Anna-
item.creatorOrcidLiese, Andreas-
item.creatorOrcidHoltmann, Dirk-
item.openairetypeArticle-
item.grantfulltextopen-
crisitem.author.deptTechnische Biokatalyse V-6-
crisitem.author.deptTechnische Biokatalyse V-6-
crisitem.author.orcid0000-0002-4867-9935-
crisitem.author.parentorgStudiendekanat Verfahrenstechnik-
crisitem.author.parentorgStudiendekanat Verfahrenstechnik-
Appears in Collections:Publications with fulltext
Files in This Item:
File Description SizeFormat
1-s2.0-S2215017X18303217-main.pdf987,21 kBAdobe PDFThumbnail
View/Open
Show simple item record

Page view(s)

142
Last Week
0
Last month
3
checked on Jul 7, 2020

Download(s)

112
checked on Jul 7, 2020

Google ScholarTM

Check

Note about this record

Export

This item is licensed under a Creative Commons License Creative Commons