Please use this identifier to cite or link to this item: https://doi.org/10.15480/882.2514
DC FieldValueLanguage
dc.contributor.authorSinnecker, Heike-
dc.contributor.authorKrause, Thorsten-
dc.contributor.authorKoelling, Sabine-
dc.contributor.authorLautenschläger, Ingmar-
dc.contributor.authorFrey, Andreas-
dc.date.accessioned2019-11-22T07:33:14Z-
dc.date.available2019-11-22T07:33:14Z-
dc.date.issued2014-11-12-
dc.identifier.citationBeilstein Journal of Nanotechnology 1 (5): 2092-2101 (2014-01-01)de_DE
dc.identifier.issn2190-4286de_DE
dc.identifier.urihttp://hdl.handle.net/11420/3845-
dc.description.sponsorshipDeutsche Forschungsgemeinschaftde_DE
dc.language.isoende_DE
dc.publisherBeilstein-Institut zur Förderung der Chemischen Wissenschaftende_DE
dc.relation.ispartofBeilstein journal of nanotechnologyde_DE
dc.rightsCC BY 2.0de_DE
dc.subjectBarrierde_DE
dc.subjectIsolated intestinede_DE
dc.subjectMucusde_DE
dc.subjectNanoparticlesde_DE
dc.subjectUptakede_DE
dc.subject.ddc540: Chemiede_DE
dc.subject.ddc610: Medizinde_DE
dc.titleThe gut wall provides an effective barrier against nanoparticle uptakede_DE
dc.typeArticlede_DE
dc.identifier.doi10.15480/882.2514-
dc.type.diniarticle-
dcterms.DCMITypeText-
tuhh.identifier.urnurn:nbn:de:gbv:830-882.055614-
tuhh.oai.showtruede_DE
tuhh.abstract.englishBackground: The omnipresence of nanoparticles (NPs) in numerous goods has led to a constant risk of exposure and inadvertent uptake for humans. This situation calls for thorough investigation of the consequences of NP intake. As the vast mucosa of the human gastrointestinal tract represents an attractive site of entry, we wanted to take a look on the fate that ingested NPs suffer in the gut. As a model to investigate NP uptake we used the isolated perfused rat small intestine. Differently sized fluorescent latex particles were used as exemplary anthropogenic NPs. Results: The particles were administered as bolus into the isolated intestine, and samples from the luminal, vascular and lymphatic compartments were collected over time. NP amounts in the different fluids were determined by fluorescence measurements. No particles could be detected in the vascular and lymphatic system. By contrast a major amount of NPs was found in luminal samples. Yet, a substantial share of particles could not be recovered in the fluid fractions, indicating a sink function of the intestinal tissue for NPs. A histological examination of the gut revealed that virtually no particles adhered to the epithelium or resided in the tissue, the bulk of particles seemed to be trapped in the mucus lining the gut tube. When this mucus was dissolved and removed from the gut almost the entire amount of particles missing could be recovered: over 95% of the given NPs were present in the two fractions, the luminal samples and the dissolved mucus. To foster NP uptake via an extended interaction time with the epithelium, the intestinal peristalsis was decelerated and the duration of the experiment was prolonged. Even under those conditions, no particle fluorescence was detected in the vascular and lymphatic samples. Conclusion: We could show that after intestinal exposure with a large dose of NPs the vast majority of NPs did obviously not come into contact with the epithelium but was either directly discarded from the gut or trapped in mucus. The healthy small intestinal tract evidently provides an effective barrier against NP uptake whereby the mucus film seems to play an important role.de_DE
tuhh.publisher.doi10.3762/bjnano.5.218-
tuhh.publication.instituteZentrallabor Chemische Analytik Lde_DE
tuhh.identifier.doi10.15480/882.2514-
tuhh.type.opus(wissenschaftlicher) Artikel-
dc.type.driverarticle-
dc.rights.cchttps://creativecommons.org/licenses/by/2.0/de_DE
dc.type.casraiJournal Article-
tuhh.container.volume5de_DE
tuhh.container.startpage2092de_DE
tuhh.container.endpage2101de_DE
dc.relation.projectPriority Program SPP 1313 NanoBioResponses (DFG Fr 958/5-1 & 5-2)de_DE
dc.rights.nationallicensefalsede_DE
local.status.inpressfalsede_DE
item.creatorOrcidSinnecker, Heike-
item.creatorOrcidKrause, Thorsten-
item.creatorOrcidKoelling, Sabine-
item.creatorOrcidLautenschläger, Ingmar-
item.creatorOrcidFrey, Andreas-
item.openairecristypehttp://purl.org/coar/resource_type/c_6501-
item.fulltextWith Fulltext-
item.openairetypeArticle-
item.creatorGNDSinnecker, Heike-
item.creatorGNDKrause, Thorsten-
item.creatorGNDKoelling, Sabine-
item.creatorGNDLautenschläger, Ingmar-
item.creatorGNDFrey, Andreas-
item.grantfulltextopen-
item.languageiso639-1en-
item.cerifentitytypePublications-
crisitem.author.deptZentrallabor Chemische Analytik L-
crisitem.author.parentorgZentrale Forschungsdienstleistung ZFD-
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