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Preparative recovery of bioactive molecules from conditioned medium of CHO-K1 cell culture
Citation Link: https://doi.org/10.15480/882.2525
Publikationstyp
Doctoral Thesis
Date Issued
2019
Sprache
English
Author(s)
Advisor
Referee
Title Granting Institution
Technische Universität Hamburg
Place of Title Granting Institution
Hamburg
Examination Date
2019-03-26
Institut
TORE-DOI
TORE-URI
Citation
Technische Universität Hamburg (2019)
This work examines the biological activity of conditioned medium on the growth, death and metabolic kinetics of CHO-K1 cell culture. A membrane adsorption chromatography method was developed for the preparative capture, recovery and fractionation of the bioactive molecules responsible for some of the determined effects. This resulted in the isolation of two fractions with biological activity when supplemented to exponentially growing cells. The results of this work suggest the interplay of two opposing signaling molecules during the batch cultivation of CHO-K1 cell culture and the possible production and accumulation of autoinhibitors previously described in the literature for hybridoma cells.
DDC Class
500: Naturwissenschaften
570: Biowissenschaften, Biologie
More Funding Information
German Federal Ministry of Education and Research (BMBF, Grant 031B222)
Deutsche Forschungsgemeinschaft (DFG; grant ZE 542/9-1).
Deutsche Forschungsgemeinschaft (DFG; grant ZE 542/9-1).
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Dissertation Endfassung.pdf
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2.34 MB
Format
Adobe PDF