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  4. Fermentative oxidation of butane in bubble column reactors
 
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Fermentative oxidation of butane in bubble column reactors

Publikationstyp
Journal Article
Publikationsdatum
2020-03-15
Sprache
English
Author
Sluyter, Gerrit 
Kleber, Joscha 
Perz, Frederic 
Grund, Bastian 
Leuchs, Susanne 
Sieberz, Simone 
Bubenheim, Paul orcid-logo
Thum, Oliver 
Liese, Andreas orcid-logo
Institut
Technische Biokatalyse V-6 
TORE-URI
http://hdl.handle.net/11420/4679
Enthalten in
Biochemical engineering journal 
Volume
155
Article Number
107486
Citation
Biochemical Engineering Journal (155): 107486 (2020-03-15)
Publisher DOI
10.1016/j.bej.2020.107486
Scopus ID
2-s2.0-85077643298
Butane is an abundant side product of oil and gas production and is mostly burned for energy and heat production. The alkane monooxygenase enzyme system AlkBGT expressed in E. coli offers a robust model system with a fast butane uptake for microbial oxidation at low temperatures (25–40 °C) and pressures (1.1–1.5 bar). The process is established in a two liter bubble column under strict safety requirements obligatory for butane/air mixtures. Detailed characterization of the transport limited process regarding the relevant process parameters gassing rate / superficial velocity (0.25–1.5 L·min−1/ 0.8 – 5 cm·s−1), butane content (14–28 % v/v), pressure as well as temperature is carried out. Classical kLa-transferability is shown inside the selected process window. The determined optimum (1.5 L·min−1/ 5 cm·s−1; 1.5 bar; 35 °C and 14 % v/v) is validated and a first scale up to 5 L is approximated. The potential is discussed as a first step to a fermentative oxidation of butane on industrial scale.
Schlagworte
Bubble column
Butane
Fermentation
Multiphase reactor
Oxidation
Transport limitation
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