Please use this identifier to cite or link to this item: https://doi.org/10.15480/882.2850
DC FieldValueLanguage
dc.contributor.authorPerz, Frederic-
dc.contributor.authorUlber, Roland-
dc.contributor.authorAlcalde, Miguel-
dc.contributor.authorBubenheim, Paul-
dc.contributor.authorHollmann, Frank-
dc.contributor.authorHoltmann, Dirk-
dc.contributor.authorLiese, Andreas-
dc.contributor.authorBormann, Sebastian-
dc.date.accessioned2020-07-29T08:41:23Z-
dc.date.available2020-07-29T08:41:23Z-
dc.date.issued2020-06-09-
dc.identifier.citationChemCatChem 14 (12): 3666-3669 (2020)de_DE
dc.identifier.issn1867-3899de_DE
dc.identifier.urihttp://hdl.handle.net/11420/6906-
dc.description.abstractUnspecific peroxygenases have recently gained significant interest due to their ability to catalyse the hydroxylation of non-activated C−H bonds using only hydrogen peroxide as a co-substrate. However, the development of preparative processes has so far mostly concentrated on benzylic hydroxylations using liquid substrates. Herein, we demonstrate the application of a peroxygenase for the hydroxylation of the inert, gaseous substrate butane to 2-butanol in a bubble column reactor. The influence of hydrogen peroxide feed rate and enzyme loading on product formation, overoxidation to butanone and catalytic efficiency is investigated at 200 mL scale. The process is scaled up to 2 L and coupled with continuous extraction. This setup allowed the production of 115 mmol 2-butanol and 70 mmol butanone with an overall total turnover number (TTN) of over 15.000, thereby demonstrating the applicability of peroxygenases for preparative hydroxylation of such inert, gaseous substrates at mild reaction conditions.en
dc.language.isoende_DE
dc.publisherWileyde_DE
dc.relation.ispartofChemCatChemde_DE
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/de_DE
dc.subjectbubble columnde_DE
dc.subjectbutanede_DE
dc.subjectbutanolde_DE
dc.subjecthydroxylationde_DE
dc.subjectperoxygenasede_DE
dc.subject.ddc570: Biowissenschaften, Biologiede_DE
dc.titleEnzymatic Oxidation of Butane to 2-Butanol in a Bubble Columnde_DE
dc.typeArticlede_DE
dc.identifier.doi10.15480/882.2850-
dc.type.diniarticle-
dcterms.DCMITypeText-
tuhh.identifier.urnurn:nbn:de:gbv:830-882.0100843-
tuhh.oai.showtruede_DE
tuhh.abstract.englishUnspecific peroxygenases have recently gained significant interest due to their ability to catalyse the hydroxylation of non-activated C−H bonds using only hydrogen peroxide as a co-substrate. However, the development of preparative processes has so far mostly concentrated on benzylic hydroxylations using liquid substrates. Herein, we demonstrate the application of a peroxygenase for the hydroxylation of the inert, gaseous substrate butane to 2-butanol in a bubble column reactor. The influence of hydrogen peroxide feed rate and enzyme loading on product formation, overoxidation to butanone and catalytic efficiency is investigated at 200 mL scale. The process is scaled up to 2 L and coupled with continuous extraction. This setup allowed the production of 115 mmol 2-butanol and 70 mmol butanone with an overall total turnover number (TTN) of over 15.000, thereby demonstrating the applicability of peroxygenases for preparative hydroxylation of such inert, gaseous substrates at mild reaction conditions.de_DE
tuhh.publisher.doi10.1002/cctc.202000431-
tuhh.publication.instituteTechnische Biokatalyse V-6de_DE
tuhh.identifier.doi10.15480/882.2850-
tuhh.type.opus(wissenschaftlicher) Artikel-
dc.type.driverarticle-
dc.type.casraiJournal Article-
tuhh.container.issue14de_DE
tuhh.container.volume12de_DE
tuhh.container.startpage3666de_DE
tuhh.container.endpage3669de_DE
dc.rights.nationallicensefalsede_DE
local.status.inpressfalsede_DE
local.funding.infoEuropean Research Commision (ERC) grant, No 648026de_DE
item.grantfulltextopen-
item.openairetypeArticle-
item.creatorOrcidPerz, Frederic-
item.creatorOrcidUlber, Roland-
item.creatorOrcidAlcalde, Miguel-
item.creatorOrcidBubenheim, Paul-
item.creatorOrcidHollmann, Frank-
item.creatorOrcidHoltmann, Dirk-
item.creatorOrcidLiese, Andreas-
item.creatorOrcidBormann, Sebastian-
item.openairecristypehttp://purl.org/coar/resource_type/c_6501-
item.fulltextWith Fulltext-
item.cerifentitytypePublications-
item.mappedtypeArticle-
item.languageiso639-1en-
item.creatorGNDPerz, Frederic-
item.creatorGNDUlber, Roland-
item.creatorGNDAlcalde, Miguel-
item.creatorGNDBubenheim, Paul-
item.creatorGNDHollmann, Frank-
item.creatorGNDHoltmann, Dirk-
item.creatorGNDLiese, Andreas-
item.creatorGNDBormann, Sebastian-
crisitem.author.deptTechnische Biokatalyse V-6-
crisitem.author.deptTechnische Biokatalyse V-6-
crisitem.author.deptTechnische Biokatalyse V-6-
crisitem.author.orcid0000-0003-4106-9576-
crisitem.author.orcid0000-0002-7674-0967-
crisitem.author.orcid0000-0001-6780-7616-
crisitem.author.orcid0000-0001-6954-4274-
crisitem.author.orcid0000-0003-4821-756X-
crisitem.author.orcid0000-0002-4867-9935-
crisitem.author.parentorgStudiendekanat Verfahrenstechnik-
crisitem.author.parentorgStudiendekanat Verfahrenstechnik-
crisitem.author.parentorgStudiendekanat Verfahrenstechnik-
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