Ohde, DanielDanielOhdeThomas, BenjaminBenjaminThomasBubenheim, PaulPaulBubenheimLiese, AndreasAndreasLiese2020-11-232020-11-232021-02Biochemical Engineering Journal (166): 107825 (2021-02)http://hdl.handle.net/11420/7900Reversible enzymatic decarboxylation reactions are known for their thermodynamic limitations regarding the carboxylation. Significant thermodynamic and kinetic improvements were achieved for the carboxylation of resorcinol by the 2,6-dihydroxybenzoic acid decarboxylase from Rhizobium sp. utilizing the amine scrubber triethanolamine for CO₂ mediation. In a first approach, maximal yields of up to 50 % are achieved and are found to correlate with the CO₂ loading capacity of triethanolamine. In a second approach, application of quaternary amines for in situ carboxylation product removal by precipitation improves the achievable yields depending on utilized amine concentration. This demonstrates the possibility for a reusable system. By variation of hydrated CO₂ species ratio and concentration, CO₂ and alternatively H₂CO₃ is identified acting as second substrate.en1369-703X2021BiotransformationCarboxylationCO fixation 2DecarboxylaseReaction mechanismTechnikJournal Article10.1016/j.bej.2020.107825Other