Hoang, Manh DatManh DatHoangDoan, Dieu ThiDieu ThiDoanKremling, AndreasAndreasKremlingHeins, Anna-LenaAnna-LenaHeins2023-11-132023-11-132022-08-25ProcessNet and DECHEMA-BioTechNet Jahrestagungen / 13th ESBES Symposium 2022https://hdl.handle.net/11420/44129Dynamic environmental conditions in industrial bioprocesses can lead to formation of population heterogeneities and thuslowered productivities. Since this phenomenon is poorly understood, an integrativeapproach of experimental exploitation andtheoretical prediction might improve comprehension. Population heterogeneity in L-phenylalanine production is experimentally studied in a fed-batch process with anEscherichia coliquadruple reporter strain.Industrial bioprocess conditions are simulated in a novel two-compartment setupcoupling a stirred-tank bioreactor to acoiled flow inverter as bypass. Fluorescence of the reporter strain revealed differences in single-cell physiology for homogeneous and dynamic cultivation conditions. The experimental data is further integrated into a theoretical description of aheterogeneous population. A coarse-grained approach is established with strictmass conservation for bacterial growthduring the fed-batch process. This iscoupled with an environmental model characterizing the bioprocess dynamics tocapture the interaction between the cellpopulation and the bioreactor.en1522-264020229Conference Presentation10.1002/cite.202255092Presentation