Lochner, AdrianeAdrianeLochnerGiannone, Richard J.Richard J.GiannoneKeller, MartinMartinKellerAntranikian, GarabedGarabedAntranikianGraham, David E.David E.GrahamHettich, Robert L.Robert L.Hettich2022-03-022022-03-022011-10-11Journal of Proteome Research 10 (12): 5302-5314 (2011)http://hdl.handle.net/11420/11804Mass spectrometric analysis of Caldicellulosiruptor obsidiansis cultures grown on four different carbon sources identified 65% of the cells' predicted proteins in cell lysates and supernatants. Biological and technical replication together with sophisticated statistical analysis were used to reliably quantify protein abundances and their changes as a function of carbon source. Extracellular, multifunctional glycosidases were significantly more abundant on cellobiose than on the crystalline cellulose substrates Avicel and filter paper, indicating either disaccharide induction or constitutive protein expression. Highly abundant flagellar, chemotaxis, and pilus proteins were detected during growth on insoluble substrates, suggesting motility or specific substrate attachment. The highly abundant extracellular binding protein COB47-0549 together with the COB47-1616 ATPase might comprise the primary ABC-transport system for cellooligosaccharides, while COB47-0096 and COB47-0097 could facilitate monosaccharide uptake. Oligosaccharide degradation can occur either via extracellular hydrolysis by a GH1 β-glycosidase or by intracellular phosphorolysis using two GH94 enzymes. When C. obsidiansis was grown on switchgrass, the abundance of hemicellulases (including GH3, GH5, GH51, and GH67 enzymes) and certain sugar transporters increased significantly. Cultivation on biomass also caused a concerted increase in cytosolic enzymes for xylose and arabinose fermentation. © 2011 American Chemical Society.en1535-390720111253025314ACS Publicationsbioenergy researchmicrobial cellulose degradationmicrobial proteomicsquantitative proteomicsthermophilic bacteriaBiowissenschaften, BiologieJournal Article10.1021/pr200536j21988591Journal Article