Gisriel, ChristopherChristopherGisrielCoe, JesseJesseCoeLetrun, RomainRomainLetrunYefanov, OleksandrOleksandrYefanovLuna-Chavez, CesarCesarLuna-ChavezStander, Natasha E.Natasha E.StanderLisova, StellaStellaLisovaMariani, ValerioValerioMarianiKuhn, ManuelaManuelaKuhnAplin, StevenStevenAplinGrant, Thomas D.Thomas D.GrantDörner, Katerina HenrikeKaterina HenrikeDörnerSato, TokushiTokushiSatoEchelmeier, AustinAustinEchelmeierCruz Villarreal, JorvaniJorvaniCruz VillarrealHunter, Mark S.Mark S.HunterWiedorn, Max OliverMax OliverWiedornKnoška, JurajJurajKnoškaMazalova, VictoriaVictoriaMazalovaRoy-Chowdhury, ShatabdiShatabdiRoy-ChowdhuryYang, Jay HowJay HowYangJones, AlexAlexJonesBean, RichardRichardBeanBielecki, JohanJohanBieleckiKim, YoonheeYoonheeKimMills, GrantGrantMillsWeinhausen, BrittaBrittaWeinhausenMeza, Jose D.Jose D.MezaAl-Qudami, NasserNasserAl-QudamiBajt, SašaSašaBajtBrehm, GerritGerritBrehmBotha, SabineSabineBothaBoukhelef, DjelloulDjelloulBoukhelefBrockhauser, SandorSandorBrockhauserBruce, Barry D.Barry D.BruceColeman, Matthew A.Matthew A.ColemanDanilevski, CyrilCyrilDanilevskiDiscianno, ErinErinDisciannoDobson, ZacharyZacharyDobsonFangohr, HansHansFangohrMartin-Garcia, Jose ManuelJose ManuelMartin-GarciaGevorkov, YaroslavYaroslavGevorkovHauf, SteffenSteffenHaufHosseinizadeh, AhmadAhmadHosseinizadehJanuschek, F.F.JanuschekKetawala, Gihan K.Gihan K.KetawalaKupitz, ChristopherChristopherKupitzMaia, LuisLuisMaiaManetti, MaurizioMaurizioManettiMesserschmidt, MarcMarcMesserschmidtMichelat, ThomasThomasMichelatMondal, JyotirmoyJyotirmoyMondalOurmazd, AbbasAbbasOurmazdPrevitali, GianpietroGianpietroPrevitaliSarrou, IosifinaIosifinaSarrouSchön, SilvanSilvanSchönSchwander, PeterPeterSchwanderShelby, Megan L.Megan L.ShelbySilenzi, AlessandroAlessandroSilenziSztuk-Dambietz, JolantaJolantaSztuk-DambietzSzuba, JanuszJanuszSzubaTurcato, MonicaMonicaTurcatoWhite, Thomas A.Thomas A.WhiteWrona, KrzysztofKrzysztofWronaXu, ChenChenXuAbdellatif, Mohamed H.Mohamed H.AbdellatifZook, James D.James D.ZookSpence, John C. H.John C. H.SpenceChapman, Henry N.Henry N.ChapmanBarty, AntonAntonBartyKirian, Richard A.Richard A.KirianFrank, MatthiasMatthiasFrankRos, AlexandraAlexandraRosSchmidt, MariusMariusSchmidtFromme, RaimundRaimundFrommeMancuso, Adrian P.Adrian P.MancusoFromme, PetraPetraFrommeZatsepin, NadiaNadiaZatsepin2019-11-152019-11-152019-11-04Nature Communications 1 (10): 5021 (2019-12-01)http://hdl.handle.net/11420/3790The world’s first superconducting megahertz repetition rate hard X-ray free-electron laser (XFEL), the European XFEL, began operation in 2017, featuring a unique pulse train structure with 886 ns between pulses. With its rapid pulse rate, the European XFEL may alleviate some of the increasing demand for XFEL beamtime, particularly for membrane protein serial femtosecond crystallography (SFX), leveraging orders-of-magnitude faster data collection. Here, we report the first membrane protein megahertz SFX experiment, where we determined a 2.9 Å-resolution SFX structure of the large membrane protein complex, Photosystem I, a > 1 MDa complex containing 36 protein subunits and 381 cofactors. We address challenges to megahertz SFX for membrane protein complexes, including growth of large quantities of crystals and the large molecular and unit cell size that influence data collection and analysis. The results imply that megahertz crystallography could have an important impact on structure determination of large protein complexes with XFELs.en2041-172320191Nature Publishing Group UKhttps://creativecommons.org/licenses/by/4.0/InformatikPhysikJournal Articleurn:nbn:de:gbv:830-882.05495410.15480/882.250610.1038/s41467-019-12955-310.15480/882.2506Journal Article