Hackbarth, MaxMaxHackbarthGescher, JohannesJohannesGescherHorn, HaraldHaraldHornReiner, Johannes EberhardJohannes EberhardReiner2023-02-132023-02-132023-02Bioresource Technology Reports 21: 101357 (2023-02)http://hdl.handle.net/11420/14809This study discusses the construction and operation of a membrane-less bioelectrochemical reactor that employs rotating working electrodes with a surface area of up to 1 m2. As a proof-of-principle for an aerobic microbial electrosynthesis process, Kyrpidia spormannii was cultivated in the reactor. Optical coherence tomography was used to examine the spatial distribution of the cathodic biofilm. After 24 days 87 % of the cathode surface was covered with biofilm that was characterized by a radial increase in its biovolume towards the circumcenter of the electrodes reaching up to 92.13 μm3 μm−2. To demonstrate the versatility of the system, we further operated the reactor as a microbial electrolysis cell employing a co-culture of Shewanella oneidensis and Geobacter sulfurreducens. Anodic current densities of up to 130 μA cm−2 were measured during these batch experiments. This resulted in a maximum production rate of 0.43 L of pure hydrogen per liter reactor volume and day.en2589-014X2023Kyrpidia spormanniiMicrobial electrolysis cellPure culture microbial electrosynthesisRotating biological contactorRotating disc bioelectrochemical reactorJournal Article10.1016/j.biteb.2023.101357Other