Huang, LeiLeiHuangRomero, ElviraElviraRomeroRessmann, Anna K.Anna K.RessmannRudroff, FlorianFlorianRudroffHollmann, FrankFrankHollmannFraaije, Marco W.Marco W.FraaijeKara, SelinSelinKara2019-07-192019-07-192017-06-19Advanced Synthesis and Catalysis 12 (359): 2142-2148 (2017-06-19)http://hdl.handle.net/11420/3012A nicotinamide adenine dinucleotide (NADH)-dependent redox-neutral convergent cascade composed of a recently discovered type II flavin-containing monooxygenase (FMO−E) and horse liver alcohol dehydrogenase (HLADH) has been established. Two model reaction cascades were analyzed for the synthesis of γ-butyrolactone and chiral bicyclic lactones. In the former cascade, all substrates were converted into one single product γ-butyrolactone with high atom efficiency. More than 130 mM γ-butyrolactone were obtained when applying 100 mM cyclobutanone and 50 mM 1,4-butanediol in this cascade. In the second cascade where bicyclo[4.2.0]octan-7-one and cis-1,2-cyclohexanedimethanol were coupled, the ketone substrate was converted to the corresponding normal lactone with an ee value of 89–74% (3aS, 7aS) by FMO−E alone and the abnormal lactone with an ee value of >99% (3aR, 7aS) was formed by both HLADH and FMO−E. (Figure presented.).en1615-415020171221422148Allgemeines, WissenschaftJournal Article10.1002/adsc.201700401Other