Keskin, SercanSercanKeskinBesztejan, StephanieStephanieBesztejanKassier, GüntherGüntherKassierManz, StephanieStephanieManzBücker, RobertRobertBückerRiekeberg, SvenjaSvenjaRiekebergTrieu, Hoc KhiemHoc KhiemTrieuRentmeister, AndreaAndreaRentmeisterMiller, R. J. DwayneR. J. DwayneMiller2020-03-082020-03-082015-11-19Journal of Physical Chemistry Letters 22 (6): 4487-4492 (2015-11-19)http://hdl.handle.net/11420/5233Base-pairing stability in DNA-gold nanoparticle (DNA-AuNP) multimers along with their dynamics under different electron beam intensities was investigated with in-liquid transmission electron microscopy (in-liquid TEM). Multimer formation was triggered by hybridization of DNA oligonucleotides to another DNA strand (Hyb-DNA) related to the concept of DNA origami. We analyzed the degree of multimer formation for a number of samples and a series of control samples to determine the specificity of the multimerization during the TEM imaging. DNA-AuNPs with Hyb-DNA showed an interactive motion and assembly into 1D structures once the electron beam intensity exceeds a threshold value. This behavior was in contrast with control studies with noncomplementary DNA linkers where statistically significantly reduced multimerization was observed and for suspensions of citrate-stabilized AuNPs without DNA, where we did not observe any significant motion or aggregation. These findings indicate that DNA base-pairing interactions are the driving force for multimerization and suggest a high stability of the DNA base pairing even under electron exposure.1948-718520152244874492Journal Article10.1021/acs.jpclett.5b02075Other