Wölfel, Eva MariaEva MariaWölfelBartsch, BenjaminBenjaminBartschKoldehoff, JasminJasminKoldehoffFiedler, Imke A. K.Imke A. K.FiedlerDragoun Kolibová, SofieSofieDragoun KolibováSchmidt, Felix NikolaiFelix NikolaiSchmidtKrug, JohannesJohannesKrugLin, Mei ChunMei ChunLinPüschel, KlausKlausPüschelOndruschka, BenjaminBenjaminOndruschkaZimmermann, Elizabeth A.Elizabeth A.ZimmermannJelitto, HansHansJelittoSchneider, Gerold A.Gerold A.SchneiderGludovatz, BerndBerndGludovatzBusse, BjörnBjörnBusse2023-12-122023-12-122023-12JBMR Plus 7 (12): e10839 (2023-12)https://hdl.handle.net/11420/44554Type 2 diabetes mellitus (T2DM) is a metabolic disease affecting bone tissue and leading to increased fracture risk in men and women, independent of bone mineral density (BMD). Thus, bone material quality (i.e., properties that contribute to bone toughness but are not attributed to bone mass or quantity) is suggested to contribute to higher fracture risk in diabetic patients and has been shown to be altered. Fracture toughness properties are assumed to decline with aging and age-related disease, while toughness of human T2DM bone is mostly determined from compression testing of trabecular bone. In this case-control study, we determined fracture resistance in T2DM cortical bone tissue from male individuals in combination with a multiscale approach to assess bone material quality indices. All cortical bone samples stem from male nonosteoporotic individuals and show no significant differences in microstructure in both groups, control and T2DM. Bone material quality analyses reveal that both control and T2DM groups exhibit no significant differences in bone matrix composition assessed with Raman spectroscopy, in BMD distribution determined with quantitative back-scattered electron imaging, and in nanoscale local biomechanical properties assessed via nanoindentation. Finally, notched three-point bending tests revealed that the fracture resistance (measured from the total, elastic, and plastic J-integral) does not significantly differ in T2DM and control group, when both groups exhibit no significant differences in bone microstructure and material quality. This supports recent studies suggesting that not all T2DM patients are affected by a higher fracture risk but that individual risk profiles contribute to fracture susceptibility, which should spur further research on improving bone material quality assessment in vivo and identifying risk factors that increase bone fragility in T2DM. © 2023 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.en2473-4039202312Wileyhttps://creativecommons.org/licenses/by/4.0/BONE MATERIAL QUALITYCORTICAL BONEFRACTURE TOUGHNESSMECHANICAL TESTINGT2DMMedicine, HealthJournal Article10.15480/882.891410.1002/jbm4.1083910.15480/882.8914Journal Article