Pätzold, MagdalenaMagdalenaPätzoldWeimer, AnnaAnnaWeimerLiese, AndreasAndreasLieseHoltmann, DirkDirkHoltmann2019-04-232019-04-232019Biotechnology report (22): e00333 (2019)http://hdl.handle.net/11420/2364The Candida rugosa lipase catalyzed esterification of (-)-menthol and lauric acid (LA) was studied in a eutectic mixture formed by both substrates((-)-menthol:LA 3:1, mol/mol). No additional reaction solvent was necessary, since the (-)-menthol:LA deep eutectic solvent (DES) acts as combined reaction medium and substrate pool. Therefore, the esterification is conducted under solvent-free conditions. The thermodynamic water activity (aw) was identified as a key parameter affecting the esterification performance in the (-)-menthol:LA DES. A response surface methodology was applied to optimize the esterification conditions in terms aw, amount of C. rugosa lipase (mCRL) and reaction temperature. Under the optimized reaction conditions (aw = 0.55; mCRL =60 mg; T =45 °C), a conversion of 95 ± 1% LA was achieved (one day), the final (-)-menthyl lauric acid ester concentration reached 1.36 ± 0.04 M (2.25 days). The experimental product formation rate agreed very well with the model prediction.en2215-017X2019e00333https://creativecommons.org/licenses/by-nc-nd/4.0/deep eutectic solventbiocatalysiswater activityreaction medium engineeringresponse surface methodologydesign of experimentsNaturwissenschaftenJournal Articleurn:nbn:de:gbv:830-882.03123110.15480/882.220510.1016/j.btre.2019.e0033310.15480/882.2205Other