Widderich, NiklasNiklasWidderichStotz, Johanna RuthJohanna RuthStotzLohkamp, FlorianFlorianLohkampVisscher, Christian FrankChristian FrankVisscherSchwaneberg, UlrichUlrichSchwanebergLiese, AndreasAndreasLieseBubenheim, PaulPaulBubenheimRuff, Anna JoelleAnna JoelleRuff2024-10-042024-10-042024-05-13Bioresources and Bioprocessing 11 (1): 49 (2024)https://hdl.handle.net/11420/49359Side streams from the milling industry offer excellent nutritional properties for animal feed; yet their use is constrained by the elevated phosphorus (P) content, mainly in the form of phytate. Biotechnological P recovery fosters sustainable P management, transforming these streams into P-depleted animal feed through enzymatic hydrolysis. The enzymatic P mobilization not only enables P recovery from milling by-products but also supports the valorization of these streams into P-depleted animal feeds. Our study presents the scalability and applicability of the process and characterizes the resulting P-depleted rye bran as animal feed component. Batch mode investigations were conducted to mobilize P from 100 g to 37.1 kg of rye bran using bioreactors up to 400 L. P reductions of 89% to 92% (reducing from 12.7 gP/kg to 1.41–1.28 gP/kg) were achieved. In addition, High Performance Ion Chromatography (HPIC) analysis showed complete depletion of phytate. The successful recovery of the enzymatically mobilized P from the process wastewater by precipitation as struvite and calcium hydrogen phosphate is presented as well, achieving up to 99% removal efficiency. Our study demonstrates a versatile process that is easily adaptable, allowing for a seamless implementation on a larger scale. Graphical Abstract: (Figure presented.).en2197-436520241Springerhttps://creativecommons.org/licenses/by/4.0/Circular phosphorus bioeconomyPhosphorus mobilizationPhosphorus recoveryPhosphorus reduced animal feedSustainabilityUp-scalingValorization of plant byproductsTechnology::660: Chemistry; Chemical Engineering::660.6: BiotechnologyJournal Article10.15480/882.1336010.1186/s40643-024-00765-510.15480/882.13360Journal Article