Johannsen, JensJensJohannsenMeyer, FrancescaFrancescaMeyerEngelmann, ClaudiaClaudiaEngelmannLiese, AndreasAndreasLieseFieg, GeorgGeorgFiegBubenheim, PaulPaulBubenheimWaluga, ThomasThomasWaluga2021-10-042021-10-042021-01-11AIChE Journal 67 (4): e17158 (2021-04)http://hdl.handle.net/11420/10430Biotechnological application of multiple enzymes in different phases for target compounds synthesis poses a significant challenge for industrial process development. At the same time, a growing demand for natural flavors and fragrances opens up possibilities for novel biotechnological processes to replace current chemical synthesis routes, with additional advantages such as avoiding harsh reaction conditions and toxic chemicals, and less by-products in the system. Within complex biotechnological processes, the key for unfolding their industrial application potential in bioprocess engineering lies in their mathematical modeling. In this contribution, a multi-enzyme cascade reaction in a two-phase system implemented in a miniplant-scale reactor setup is mathematically modeled for the example of the flavoring agent cinnamyl cinnamate. Using our validated model and a mathematical optimization tool based on a genetic algorithm, optimization runs are performed to demonstrate the potential of computer-aided process development for complex biotechnological processes.en1547-590520214Wileyhttps://creativecommons.org/licenses/by/4.0/cascade reactionsenzymesmathematical modelingoptimizationprocess integrationsimulationBiowissenschaften, BiologieJournal Article10.15480/882.380110.1002/aic.1715810.15480/882.3801Journal Article