Madjarov, JoanaJoanaMadjarovProkhorova, AnnaAnnaProkhorovaMessinger, ThorstenThorstenMessingerGescher, JohannesJohannesGescherKerzenmacher, SvenSvenKerzenmacher2022-12-152022-12-152016-09-04Bioresource Technology 221: 165-171 (2016-12-01)http://hdl.handle.net/11420/14367In this study, different inoculation strategies for continuously operated microbial anodes are analyzed and compared. After 20 days of operation with municipal wastewater anodes pre-incubated with a biofilm of the exoelectrogenic species Geobacter and Shewanella showed current densities of (65 ± 8) μA/cm2. This is comparable to the current densities of non-inoculated anodes and anodes inoculated with sewage sludge. Analysis of the barcoded pre-grown multispecies biofilms reveal that 99% of the original biofilm was detached after 20 days of operation with municipal wastewater. This is in contrast to previous experiments where a pre-grown biofilm of exoelectrogens was operated in batch mode. To implement pre-grown biofilms in continuous systems it will thus be necessary to reveal a window of process parameters in which typical exoelectrogenic microorganisms including model organisms can be kept and/or enriched on anodes.en1873-29762016165171Elsevier ScienceAnodeGeobacterInoculationMicrobial communityMicrobial fuel cellPregrown biofilmShewanellaWastewaterBiowissenschaften, BiologieTechnikJournal Article10.1016/j.biortech.2016.09.00427639235Other