Latus, SarahSarahLatusKulas, MaricaMaricaKulasSprenger, JohannaJohannaSprengerBhattacharya, DebayanDebayanBhattacharyaBreda, Philippe C.Philippe C.BredaWittig, LukasLukasWittigEixmann, TimTimEixmannHüttmann, GereonGereonHüttmannMaack, LennartLennartMaackEggert, DennisDennisEggertBetz, Christian StephanChristian StephanBetzSchlaefer, AlexanderAlexanderSchlaefer2024-05-312024-05-312024Progress in Biomedical Optics and Imaging - Proceedings of SPIE: 1292809 (2024)978-1-5106-7160-7978-1-5106-7161-4https://hdl.handle.net/11420/47683The increasing incidence of laryngeal carcinomas requires approaches for early diagnosis and treatment. In clinical practice, white light endoscopy of the laryngeal region is typically followed by biopsy under general anesthesia. Thus, image based diagnosis using optical coherence tomography (OCT) has been proposed to study sub-surface tissue layers at high resolution. However, accessing the region of interest requires robust miniature OCT probes that can be forwarded through the working channel of a laryngoscope. Typically, such probes generate A-scans, i.e., single column depth images, which are rather difficult to interpret. We propose a novel approach using the endoscopic camera images to spatially align these A-scans. Given the natural tissue motion and movements of the laryngoscope, the resulting OCT images show a three-dimensional representation of the sub-surface structures, which is simpler to interpret. We present the overall imaging setup and the motion tracking method. Moreover, we describe an experimental setup to assess the precision of the spatial alignment. We study different tracking templates and report root-mean-squared errors of 0.08mm and 0.18mm for sinusoidal and freehand motion, respectively. Furthermore, we also demonstrate the in-vivo application of the approach, illustrating the benefit of spatially meaningful alignment of the A-scans to study laryngeal tissue.enImage based trackingLaryngeal carcinomaMotion compensationOptical coherence tomographyComputer Science, Information and General Works::005: Computer Programming, Programs, Data and SecurityTechnology::610: Medicine, HealthConference Paper10.1117/12.3006729Conference Paper