Benito-Román, ÓscarÓscarBenito-RománAlonso, EstherEstherAlonsoGairola, KrishanKrishanGairolaCocero, Maria José AlonsoMaria José AlonsoCocero2020-06-292020-06-292013-07-18Journal of Supercritical Fluids (82): 122-128 (2013)http://hdl.handle.net/11420/6491β-Glucans were successfully extracted from different cereals (barley, de-starched barley and wheat bran) in a fixed-bed extractor using pressurized hot water (PHW) as solvent, in the range of 110-175 C, at 20 bar. Results were evaluated in terms of extraction yield, molecular weight of the extracted β-glucans and products of hydrolysis. Extraction yield was maximized at 155 C, 20 bar and 4 g/min of PHW for the three raw materials. Under these conditions, almost 100% of the initial β-glucans were extracted after 105 min of operation, and the molecular weight of the extracted β-glucans was much higher (up to 500 kDa vs. 180 kDa) compared with batch operation, revealing the feasibility of the use of fixed-bed extractors for the β-glucan extraction. Under these conditions no degradation products, such as HMF, were detected in the extract. Experimental results have revealed that the operation in fixed-bed is adequate for the extraction and fractionation of high molecular weight β-glucans in a single step operation. © 2013 Elsevier B.V. All rights reserved.en0896-84462013122128Elsevier Scienceβ-GlucanBarleyExtractionPolysaccharidePressurized hot waterIngenieurwissenschaftenJournal Article10.1016/j.supflu.2013.07.003Other