Zott, Fabian L.Fabian L.ZottKorotenko, VasiliiVasiliiKorotenkoZipse, HendrikHendrikZipse2025-05-142025-05-142022-04-05ChemBioChem 23 (7): e202100651 (2022)https://hdl.handle.net/11420/555785-Formylcytosine is an important nucleobase in epigenetic regulation, whose hydrate form has been implicated in the formation of 5-carboxycytosine as well as oligonucleotide binding events. The hydrate content of 5-formylcytosine and its uracil derivative has now been quantified using a combination of NMR and mass spectroscopic measurements as well as theoretical studies. Small amounts of hydrate can be identified for the protonated form of 5-formylcytosine and for neutral 5-formyluracil. For neutral 5-formylcytosine, however, direct detection of the hydrate was not possible due to its very low abundance. This is in full agreement with theoretical estimates.en1439-763320227Wileyhttps://creativecommons.org/licenses/by/4.0/aldehyde hydrates | computational chemistry | DNA methylation | epigenetics | modified nucleic acids | TET enzymesNatural Sciences and Mathematics::541: Physical; TheoreticalNatural Sciences and Mathematics::572: BiochemistryTechnology::610: Medicine, HealthJournal Articlehttps://doi.org/10.15480/882.1516310.1002/cbic.20210065110.15480/882.15163Journal Article