Kaesler, InesInesKaeslerGraeber, IngeborgIngeborgGraeberBorchert, Martin S.Martin S.BorchertPape, ThomasThomasPapeDieckmann, RalfRalfDieckmannDöhren, Hans vonHans vonDöhrenNielsen, PrebenPrebenNielsenLurz, RudiRudiLurzMichaelis, WalterWalterMichaelisSzewzyk, UlrichUlrichSzewzyk2020-01-212020-01-212008-08-01International Journal of Systematic and Evolutionary Microbiology 8 (58): 1815-1820 (2008)http://hdl.handle.net/11420/4431The bacterial strain Gp₄₇.1T, isolated from the marine sponge Isops phlegraei collected at the Sula Ridge off the Norwegian coast, was characterized. The isolate was a motile spirillum that was monopolarly and monotrichously flagellated. It was aerobic, Gram-negative, oxidase-positive and catalase-negative. Optimal growth occurred between 20 and 30 °C, at pH 7-8 and with a salt concentration of 2-3% (w/v). The isolate showed a relatively restricted nutritional profile. Substrate utilization tests were only positive for arabinose. Enzyme tests were positive for esterase lipase C8, lipase C14, leucine arylamidase and naphthol-AS-BI-phosphohydrolase. The strain was not able to reduce nitrate. The major cellular fatty acids were C16:1ω7 and C16:0. The DNA G+C content was 62.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparison classified the strain as a member of the order Oceanospirillales in the class Gammaproteobacteria. Strain Gp₄₇.1T formed a distinct phyletic line with less than 94% 16S rRNA gene sequence similarity to its closest relatives with validly published names. Based on the determined data, it is proposed that the strain represents a novel species in a new genus, Spongiispira norvegica gen. nov., sp. nov.; the type strain of Spongiispira norvegica is Gp₄₇.1T (=DSM 17749T =NCIMB 14401T). © 2008 IUMS.en1465-21022008818151820SGMBiowissenschaften, BiologieJournal Article10.1099/ijs.0.65439-0Other