Antonelli, AntonellaAntonellaAntonelliSzwargulski, PatrykPatrykSzwargulskiScarpa, Emanuele SalvatoreEmanuele SalvatoreScarpaThieben, FlorianFlorianThiebenGruettner, CordulaCordulaGruettnerAmbrosi, GianlucaGianlucaAmbrosiGuidi, LorettaLorettaGuidiLudewig, PeterPeterLudewigKnopp, TobiasTobiasKnoppMagnani, MauroMauroMagnani2020-04-172020-04-172020-04-01Nanomedicine 8 (15): 739-753 (2020-04-01)http://hdl.handle.net/11420/5768Aim: Magnetic particle imaging (MPI) is highly promising for biomedical applications, but optimal tracers for MPI, namely superparamagnetic iron oxide-based contrast agents, are still lacking. Materials & methods: The encapsulation of commercially available nanoparticles, specifically synomag®-D and perimag®, into human red blood cells (RBCs) was performed by a hypotonic dialysis and isotonic resealing procedure. The amounts of superparamagnetic iron oxide incorporated into RBCs were determined by Fe quantification using nuclear magnetic resonance and magnetic particle spectroscopy. Results: Perimag-COOH nanoparticles were identified as the best nanomaterial for encapsulation in RBCs. Perimag-COOH-loaded RBCs proved to be viable cells showing a good magnetic particle spectroscopy performance, while the magnetic signal of synomag-D-COOH-loaded RBCs dropped sharply. Conclusion: Perimag-COOH-loaded RBCs could be a potential tool for MPI diagnostic applications.en1748-696320208739753intravascular contrast agentsMPS/MPINMRRBCsSPIO-loaded RBCsTEMJournal Article10.2217/nnm-2019-0449Other