Ohde, DanielDanielOhdeThomas, BenjaminBenjaminThomasBubenheim, PaulPaulBubenheimLiese, AndreasAndreasLiese2024-01-182024-01-182024-01Processes 12 (1): 10 (2024-01)https://hdl.handle.net/11420/45138For the enzymatic carboxylation of resorcinol to 2,6-dihydroxybenzoic acid (2,6-DHBA) using gaseous CO2 in an aqueous triethanolamine phase, an adsorption-based in situ product removal was demonstrated. The aim is to improve the reaction yield, which is limited by an unfavourable thermodynamic equilibrium. First, a screening for a high-affinity adsorber was carried out. Then, the application of a suitable adsorber was successfully demonstrated. This enabled achieving reaction yields above 80% using the adsorber for in situ product removal. The applied biotransformation was scaled up to 1.5 L at lab-scale. Furthermore, a downstream process based on the elution and purification of the product bound to the adsorber was developed to obtain 2,6-DHBA in high purity. Recycling is one of the key factors in this system, making it possible to recycle the reaction medium, the adsorber and the solvents in additional batches.en2227-971720241MDPIhttps://creativecommons.org/licenses/by/4.0/Chemical EngineeringJournal Article10.15480/882.905810.3390/pr1201001010.15480/882.9058Journal Article