Schultz, JanaJanaSchultzScherzinger, MarvinMarvinScherzingerElbanhawy, Amr Y.Amr Y.ElbanhawyKaltschmitt, MartinMartinKaltschmitt2025-07-042025-07-042025-06-17Bioenergy Research 18 (1): 58 (2025)https://hdl.handle.net/11420/56051Major challenges in using lignocellulosic residues as biogas substrates arise from their high diversity and their typically low nitrogen content, which may not provide sufficient nitrogen for the microorganisms. To investigate to what extent such substrates can be used in biogas plants without extensive pre-treatment, this study presents a 300-day continuous co-digestion of lignocellulosic substrates (i.e., sugarcane reed, lemon, and grape leaves) and goat manure while continuously monitoring various process parameters. The results suggest a stable and effective biogas production at a carbon-to-nitrogen ratio (C/N ratio) of 33, with a production of 244 ± 15 mL<inf>N</inf> g<inf>VS</inf>⁻¹ d⁻¹ biogas. At a higher C/N ratio of 43, the process remained stable, but hindrance was encountered. Process failure occurred at a C/N ratio of 52, where a rapid decline in biogas production was observed, accompanied by an increase in the volatile fatty acids to total alkalinity ratio (from < 0.2 to 0.9), a drop in the pH-value (from > 7 to 5.4), and an increased CO<inf>2</inf>-content of the provided biogas (from > 50% to 43%). The compositional analysis of the digestate suggests an insufficient N-supply and a failure of the carbonate and ammonium buffer systems inside the reactor. The experiment also served to validate a previously developed model based on the individual substrates’ degradation kinetics. With a relative root mean square error rRMSE of 8%, the model adequately predicted biogas production within defined limits. However, it could not anticipate process breakdown at high C/N ratios, highlighting a strong limitation.en1939-124220251https://creativecommons.org/licenses/by/4.0/Agricultural residues | Biogas | Biomethane | Digestate | Lignocellulosic biomass | ModelingTechnology::600: TechnologyJournal Articlehttps://doi.org/10.15480/882.1534510.1007/s12155-025-10858-410.15480/882.15345Journal Article