Richter, KatrinKatrinRichterGescher, JohannesJohannesGescher2023-01-242023-01-242012-11-21Biochemical Society Transactions 40 (6): 1222-1226 (2012-12-01)http://hdl.handle.net/11420/14625Heterologous multiprotein expression is the tool to answer a number of questions in basic science as well as to convert strains into producers and/or consumers of certain compounds in applied sciences. Multiprotein expression can be driven by plasmids with the disadvantages that the gene dosage might, in some cases, lead to toxic effects and that the continuous addition of antibiotics is undesirable. Stable genomic expression of proteins can forgo these problems and is a helpful and promising tool in synthetic biology. In the present paper, we provide an extract of methods from the toolbox for chromosome-based heterologous expression in Escherichia coli. ©The Authors Journal compilation.en1470-87522012612221226Portland PressCounter-selectionEscherichia coliHeterologous multiprotein expressionPhagePromoterRecombineeringBiowissenschaften, BiologieConference Paper10.1042/BST2012014323176458Other