Chikere, Anthony ChibuikeAnthony ChibuikeChikereGalunsky, BorisBorisGalunskyOvermeyer, AndréAndréOvermeyerBrunner, GerdGerdBrunnerKasche, VolkerVolkerKasche2024-07-192024-07-192000-11Biotechnology Letters 22 (22): 1815-1821 (2000-11)https://hdl.handle.net/11420/48460The activity of immobilised soybean lipoxygenase-1 (LOX-1) was studied in aqueous and supercritical carbon dioxide (SCCO2) media for the production of 13S-hydroperoxyoctadecadenoic acid (13S-HPODE). In SCCO2, it was optimal at 33 °C and 25 MPa. A higher space-time yield of 5.7 x 10-3 Ms-1 mg-1 LOX-1 for 13S-HPODE was obtained in SCCO2 compared to only 5 x 10-5 Ms-1 mg-1 LOX-1 in aqueous medium. The stability of immobilised LOX-1 was only significantly affected by the pressurisation and depressurisation steps during reactions in SCCO2.en1573-677620002218151821Kluwer Academic PressImmobilised soybean lipoxygenase-1Supercritical carbon dioxideNatural Sciences and Mathematics::570: Life Sciences, BiologyJournal Article10.1023/A:1005626806321Journal Article