Sané, SabineSabineSanéRichter, KatrinKatrinRichterRubenwolf, StefanieStefanieRubenwolfMatschke, Nina JoanNina JoanMatschkeJolivalt, ClaudeClaudeJolivaltMadzak, CatherineCatherineMadzakZengerle, RolandRolandZengerleGescher, JohannesJohannesGescherKerzenmacher, SvenSvenKerzenmacher2023-01-132023-01-132014-02-17Bioresource Technology 158: 231-238 (2014)http://hdl.handle.net/11420/14570The feasibility to apply crude culture supernatants that contain the multicopper oxidases laccase or copper efflux oxidase (CueO) as oxygen reducing catalysts in a biofuel cell cathode is shown. As enzyme-secreting recombinant planktonic microorganisms, the yeast Yarrowia lipolytica and the bacterium Escherichia coli were investigated. The cultivation and operation conditions (choice of medium, pH) had distinct effects on the electro-catalytic performance. The highest current density of 119±23μAcm-2 at 0.400V vs. NHE was obtained with the crude culture supernatant of E. coli cells overexpressing CueO and tested at pH 5.0. In comparison, at pH 7.4 the electrode potential at 100μAcm-2 is 0.25V lower. Laccase-containing supernatants of Y. lipolytica yielded a maximum current density of 6.7±0.4μAcm-2 at 0.644V vs. NHE. These results open future possibilities to circumvent elaborate enzyme purification procedures and realize cost effective and easy-to-operate enzymatic biofuel cells.en1873-29762014231238Elsevier ScienceBioelectrochemistryBiofuel cellCathodeCopper efflux oxidaseLaccaseBiowissenschaften, BiologieTechnikJournal Article10.1016/j.biortech.2014.02.03824607459Other