Bastos, EduardoEduardoBastosKehili, MounaMounaKehiliKienast, MaxMaxKienastLenzen, AntoninAntoninLenzenZetzl, CarstenCarstenZetzlSmirnova, IrinaIrinaSmirnova2022-11-152022-11-152021-05-0618th European Meeting on Supercritical Fluids (EMSF 2021)http://hdl.handle.net/11420/14066The Institute for Thermal Separation Processes has developed a biochemical high pressure process cascade to produce high purity lignin from coffee silverskin (CSS) wastes. The whole process was designed to be environmentally friendly and cost efficient by only using water, enzymes and Carbon Dioxide during consecutive steps. Lipids are extracted during Supercritical CO2 extraction and proteins during the first Liquid-HotWater (LHW) Hydrolysis step. Subsequently sugars are converted from carbohydrates during a more severe hydrolysis (LHW hydrolysis at higher temperatures resp. steam explosion) and enzymatic treatment. Consequently, the lignin has been purified by a consecutive fractionation of lipids, proteins and carbohydrates from CSS. The high pressure cascade is now permanently in operation to facilitate lignin purification from any other type of lignocellulosic biomass.enCoffee by-productsadded valueSupercritical CO 2Liquid hot water hydrolysisEnzymatic hydrolysisLigninIngenieurwissenschaftenConference Poster not in Proceedingshttps://emsf2020.sciencesconf.org/Instituto Superior de Engenharia do PortoThermische Verfahrenstechnik V-8Technische Fakultät, Universität BielefeldBioMP Biomasse Hochdruck GmbH, HamburgOther