Merkel, LarsLarsMerkelSchauer, MelinaMelinaSchauerAntranikian, GarabedGarabedAntranikianBudisa, NediljkoNediljkoBudisa2019-12-062019-12-062010-07-19ChemBioChem 11 (11): 1505-1507 (2010)http://hdl.handle.net/11420/3957(Chemical Equation Presented) Nonstick proteins? We report the successful residue-specific fluorination of 24 positions in a lipase by simultaneous incorporation of three different noncanonical amino acids. The enzyme produced in a polyauxotrophic E. coli strain structurally tolerates all fluorine atoms and retains its catalytic activity very well. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.en1439-763320101115051507Wiley-VCH VerlagEnzyme activityLipasesNoncanonical amino acidsProtein engineeringSense-codon reassignmentBiowissenschaften, BiologieJournal Article10.1002/cbic.201000295Other